通过给予兔骨髓干细胞增强骨表面与骨膜之间的界面逐渐扩展来实现成骨。
Osteogenesis by gradually expanding the interface between bone surface and periosteum enhanced by bone marrow stem cell administration in rabbits.
作者信息
Sato Koichiro, Haruyama Naoto, Shimizu Yoshinaka, Hara Junichi, Kawamura Hiroshi
机构信息
Division of Maxillofacial Surgery, Department of Oral Medicine and Surgery, Tohoku University Graduate School of Dentistry, Sendai, Japan.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Jul;110(1):32-40. doi: 10.1016/j.tripleo.2009.11.005. Epub 2010 Feb 26.
OBJECTIVE
We investigated whether administration of mesenchymal stem cells (MSCs) promotes bone formation at the gap created by periosteal distraction.
STUDY DESIGN
A mesh plate was placed subperiosteally in rabbit parietal bones. Following elevation of the mesh plate, rabbit MSCs were administered into the gap. Controls received phosphate-buffered saline (PBS). The volume, height, bone mineral density (BMD), and bone mineral content (BMC) of newly formed bone were examined using microcomputed tomography. Histological analysis was performed by hematoxylin and eosin staining and immunohistochemistry for type I collagen and osteocalcin.
RESULTS
The experimental group showed significantly increased volume, height, BMD, and BMC in newly formed bone tissues at the gaps compared with the control group (P < .05). The newly formed bone tissues showed both type I collagen and osteocalcin expression in the MSC-administration group.
CONCLUSION
Mesenchymal stem cell administration may be useful to induce osteogenesis at sites of periosteal distraction.
目的
我们研究了间充质干细胞（MSCs）的施用是否能促进骨膜牵张所形成间隙处的骨形成。
研究设计
将一块网板置于兔顶骨的骨膜下。在提起网板后，将兔间充质干细胞施用于间隙处。对照组接受磷酸盐缓冲盐水（PBS）。使用微型计算机断层扫描检查新形成骨的体积、高度、骨密度（BMD）和骨矿物质含量（BMC）。通过苏木精和伊红染色以及I型胶原蛋白和骨钙素的免疫组织化学进行组织学分析。
结果
与对照组相比，实验组间隙处新形成骨组织的体积、高度、BMD和BMC显著增加（P < 0.05）。在间充质干细胞施用组中，新形成的骨组织同时显示I型胶原蛋白和骨钙素表达。
结论
施用间充质干细胞可能有助于在骨膜牵张部位诱导成骨。
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