Department of Biomedical Engineering, National Cheng Kung University, Tainan City, Taiwan.
J Agric Food Chem. 2010 Mar 24;58(6):3859-66. doi: 10.1021/jf100530c.
An alcoholic fatty liver disease was induced by drinking water containing 20% (w/w) alcohol. Therapeutic groups were orally administrated dosages of 0.25 g silymarin/kg body weight (BW) and a low dosage of Niuchangchih (Antrodia camphorata) (0.025 g/kg BW) and a high dosage of Niuchangchih (0.1 g/kg BW) per day. Niuchangchih, especially at the high dosage, not only showed a hypercholesterolemic effect (p < 0.05) but also reduced (p < 0.05) hepatic lipids in alcohol-fed rats. Those beneficial effects could be partially attributed to higher (p < 0.05) fecal cholesterol and bile acid outputs, as well as downregulations (p < 0.05) of 3-hydroxy-3-methylglutaryl-CoA reductase, sterol regulatory element-binding protein-1c, acetyl-CoA carboxylase, fatty acid synthase, and malic enzyme gene expressions; meanwhile, there was an upregulation of low-density lipoprotein receptor and peroxisome proliferator-activated alpha gene expression. Besides, Niuchangchih also enhanced (p < 0.05) the liver glutathione, Trolox equivalent antioxidant capacity, and activities of superoxide dismutase, catalase, and glutathione peroxidase and decreased the liver malondialdehyde content, which also partially contributed to the lowered (p < 0.05) serum aspartate aminotransferase levels and no observed lesion in the histological examination of alcohol-fed rats.
采用含 20%（w/w）酒精的水诱导酒精性脂肪肝疾病。治疗组每天口服给予水飞蓟素 0.25 g/kg 体重（BW）和低剂量牛樟芝（樟芝）（0.025 g/kg BW）以及高剂量牛樟芝（0.1 g/kg BW）。牛樟芝，尤其是高剂量，不仅显示出高胆固醇血症作用（p < 0.05），而且还降低了酒精喂养大鼠的肝脂质（p < 0.05）。这些有益作用部分归因于更高的（p < 0.05）粪便胆固醇和胆汁酸排泄，以及 3-羟-3-甲基戊二酰辅酶 A 还原酶、固醇调节元件结合蛋白-1c、乙酰辅酶 A 羧化酶、脂肪酸合酶和苹果酸酶基因表达下调；同时，低密度脂蛋白受体和过氧化物酶体增殖物激活受体-α基因表达上调。此外，牛樟芝还增强了（p < 0.05）肝脏谷胱甘肽、Trolox 当量抗氧化能力以及超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性，并降低了肝脏丙二醛含量，这也部分有助于降低（p < 0.05）血清天冬氨酸转氨酶水平和酒精喂养大鼠组织学检查中未观察到病变。
