Research Area of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, A-1060 Vienna, Austria.
J Zhejiang Univ Sci B. 2010 Mar;11(3):151-68. doi: 10.1631/jzus.B1000007.
Conidial fungi or molds and mildews are widely used in modern biotechnology as producers of antibiotics and other secondary metabolites, industrially important enzymes, chemicals and food. They are also important pathogens of animals including humans and agricultural crops. These various applications and extremely versatile natural phenotypes have led to the constantly growing list of complete genomes which are now available. Functional genomics and proteomics widely exploit the genomic information to study the cell-wide impact of altered genes on the phenotype of an organism and its function. This allows for global analysis of the information flow from DNA to RNA to protein, but it is usually not sufficient for the description of the global phenotype of an organism. More recently, Phenotype MicroArray (PM) technology has been introduced as a tool to characterize the metabolism of a (wild) fungal strain or a mutant. In this article, we review the background of PM applications for fungi and the methodic requirements to obtain reliable results. We also report examples of the versatility of this tool.
分生孢子真菌或霉菌和真菌广泛应用于现代生物技术中，作为抗生素和其他次生代谢物、工业上重要的酶、化学品和食品的生产者。它们也是包括人类和农业作物在内的动物的重要病原体。这些各种应用和极其多样的自然表型导致了完整基因组的不断增长列表，这些基因组现在都可以获得。功能基因组学和蛋白质组学广泛利用基因组信息来研究改变基因对生物体表型及其功能的全细胞影响。这允许从 DNA 到 RNA 到蛋白质的信息流的全局分析，但通常不足以描述生物体的全局表型。最近，表型微阵列 (PM) 技术已被引入作为一种工具来描述（野生）真菌菌株或突变体的代谢。在本文中，我们回顾了 PM 在真菌中的应用背景和获得可靠结果的方法学要求。我们还报告了该工具多功能性的示例。
