Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford OX1 3QU, UK.
Mass Spectrom Rev. 2011 Jan-Feb;30(1):1-100. doi: 10.1002/mas.20265.
This review is the fourth update of the original review, published in 1999, on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2006. The review covers fundamental studies, fragmentation of carbohydrate ions, method developments, and applications of the technique to the analysis of different types of carbohydrate. Specific compound classes that are covered include carbohydrate polymers from plants, N- and O-linked glycans from glycoproteins, glycated proteins, glycolipids from bacteria, glycosides, and various other natural products. There is a short section on the use of MALDI-TOF mass spectrometry for the study of enzymes involved in glycan processing, a section on industrial processes, particularly the development of biopharmaceuticals and a section on the use of MALDI-MS to monitor products of chemical synthesis of carbohydrates. Large carbohydrate-protein complexes and glycodendrimers are highlighted in this final section.
这是对 1999 年发表的原始综述的第四次更新，主要介绍 MALDI 质谱在碳水化合物和糖缀合物分析中的应用，文献的截止时间为 2006 年底。综述涵盖了基础研究、糖离子的碎裂、方法的发展，以及该技术在分析不同类型碳水化合物中的应用。具体涵盖的化合物类别包括植物来源的碳水化合物聚合物、糖蛋白中的 N-和 O-连接聚糖、糖化蛋白、细菌来源的糖脂、糖苷和各种其他天然产物。还有一小节介绍了 MALDI-TOF 质谱在聚糖加工酶研究中的应用，以及工业过程的应用，特别是生物制药的开发，以及 MALDI-MS 在监测碳水化合物化学合成产物中的应用。在最后一节中重点介绍了大型碳水化合物-蛋白质复合物和糖树状聚合物。
