Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK.
Mass Spectrom Rev. 2012 Mar-Apr;31(2):183-311. doi: 10.1002/mas.20333. Epub 2011 Aug 17.
This review is the fifth update of the original review, published in 1999, on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2008. The first section of the review covers fundamental studies, fragmentation of carbohydrate ions, use of derivatives and new software developments for analysis of carbohydrate spectra. Among newer areas of method development are glycan arrays, MALDI imaging and the use of ion mobility spectrometry. The second section of the review discusses applications of MALDI MS to the analysis of different types of carbohydrate. Specific compound classes that are covered include carbohydrate polymers from plants, N- and O-linked glycans from glycoproteins, biopharmaceuticals, glycated proteins, glycolipids, glycosides and various other natural products. There is a short section on the use of MALDI mass spectrometry for the study of enzymes involved in glycan processing and a section on the use of MALDI MS to monitor products of the chemical synthesis of carbohydrates with emphasis on carbohydrate-protein complexes and glycodendrimers. Corresponding analyses by electrospray ionization now appear to outnumber those performed by MALDI and the amount of literature makes a comprehensive review on this technique impractical. However, most of the work relating to sample preparation and glycan synthesis is equally relevant to electrospray and, consequently, those proposing analyses by electrospray should also find material in this review of interest.
这是对原始综述的第五次更新，该综述于 1999 年首次发表，主题为 MALDI 质谱在碳水化合物和糖缀合物分析中的应用，涵盖的文献截止到 2008 年底。综述的第一部分涵盖了基础研究、糖离子的碎裂、衍生物的应用以及新软件的开发，用于分析糖谱。方法开发的较新领域包括糖基阵列、MALDI 成像以及离子淌度谱的应用。综述的第二部分讨论了 MALDI MS 在分析不同类型碳水化合物中的应用。涵盖的特定化合物类别包括植物中的碳水化合物聚合物、糖蛋白中的 N-和 O-连接聚糖、生物制药、糖化蛋白、糖脂、糖苷和各种其他天然产物。还有一小节介绍了 MALDI 质谱在聚糖加工相关酶研究中的应用，以及 MALDI MS 用于监测碳水化合物化学合成产物的应用，重点介绍碳水化合物-蛋白质复合物和糖树状聚合物。目前，电喷雾电离的相关分析似乎比 MALDI 更为常见，而且文献数量使得对该技术进行全面综述变得不切实际。然而，与样品制备和糖合成相关的大部分工作与电喷雾同样相关，因此，那些提议进行电喷雾分析的人也应该会对本综述中与糖分析相关的内容感兴趣。
