Nuffield Department of Medicine, Target Discovery Institute, University of Oxford, Oxford, UK.
Mass Spectrom Rev. 2023 Jan;42(1):227-431. doi: 10.1002/mas.21721. Epub 2021 Oct 31.
This review is the tenth update of the original article published in 1999 on the application of matrix-assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2018. Also included are papers that describe methods appropriate to glycan and glycoprotein analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, new methods, matrices, derivatization, MALDI imaging, fragmentation and the use of arrays. The second part of the review is devoted to applications to various structural types such as oligo- and poly-saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Most of the applications are presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and highlights the impact that MALDI imaging is having across a range of diciplines. MALDI is still an ideal technique for carbohydrate analysis and advancements in the technique and the range of applications continue steady progress.
这是对 1999 年发表的原始文章的第十次更新，该文章介绍了基质辅助激光解吸/电离质谱（MALDI）质谱在碳水化合物和糖缀合物分析中的应用，并涵盖了截至 2018 年底的文献。本文还介绍了通过 MALDI 适当分析聚糖和糖蛋白的方法，例如样品制备技术，即使离子化方法不是 MALDI。综述的第一部分涵盖了一般方面，如 MALDI 过程的理论、新方法、基质、衍生化、MALDI 成像、碎片化和阵列的使用。综述的第二部分专门讨论了各种结构类型的应用，如寡糖和多糖、糖蛋白、糖脂、糖苷和生物制药。大多数应用都以表格形式呈现。综述的第三部分涵盖了该技术在医学和工业中的应用、酶反应研究以及在化学合成中的应用。报告的工作表明，越来越多地使用离子淌度等新技术，并强调了 MALDI 成像在一系列学科中的影响。MALDI 仍然是碳水化合物分析的理想技术，技术的进步和应用范围的扩大都在稳步推进。
