基于金颗粒线传感器的图案化多重病原体 DNA 检测。

Patterned multiplex pathogen DNA detection by Au particle-on-wire SERS sensor.

机构信息

Department of Chemistry, KAIST, Daejeon, Korea.

出版信息

Nano Lett. 2010 Apr 14;10(4):1189-93. doi: 10.1021/nl1000086.

PMID:20222740

Abstract

A Au particle-on-wire system that can be used as a specific, sensitive, and multiplex DNA sensor is developed. A pattern formed by multiple Au nanowire sensors provides positional address and identification for each sensor. By using this system, multiplex sensing of target DNAs was possible in a quantitative manner with a detection limit of 10 pM. Target DNAs from reference bacteria and clinical isolates were successfully identified by this sensor system, enabling diagnostics for infectious diseases.

摘要

研制了一种可作为特定、敏感和多重 DNA 传感器的金纳米线传感器系统。由多个金纳米线传感器形成的图案为每个传感器提供位置地址和识别信息。通过使用该系统，可以以 10 pM 的检测限定量进行目标 DNA 的多重传感。该传感器系统成功地识别了参考细菌和临床分离物中的目标 DNA，从而实现了传染病的诊断。