Division of Neurobiology and Ageing, Department of Zoology, Sacred Heart College, Kochi 682013, India.
Cell Stress Chaperones. 2010 Sep;15(5):743-51. doi: 10.1007/s12192-010-0177-y. Epub 2010 Mar 13.
Antioxidants are free radical scavengers and protect living organisms against oxidative damage to tissues. Experimental evidence implicates oxygen-derived free radicals as important causative agents of aging and the present study was designed to evaluate the age-related effects of deprenyl on the antioxidant defense in the cerebellum of male Wistar rats. Experimental rats of three age groups (6, 12, and 18 months old) were administered with liquid deprenyl (2 mg/kg body weight/day for a period of 15 days i.p) and levels of diagnostic marker enzymes (alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and creatine phosphokinase) in plasma, lipid peroxides, reduced glutathione and activities of glutathione-dependent antioxidant enzymes (glutathione peroxidase and glutathione-S-transferase) and antiperoxidative enzymes (catalase and superoxide dismutase) in the cerebellar tissue were determined. Intraperitonial administration of deprenyl (2 mg/kg body weight/day for a period of 15 days) significantly (p < 0.05) attenuated the age-related alterations noted in the levels of diagnostic marker enzymes plasma of experimental animals. Deprenyl also exerted an antioxidant effect against aging process by hindering lipid peroxidation to an extent. Moderate rise in the levels of reduced glutathione and activities of glutathione-dependent antioxidant enzymes and antiperoxidative enzymes was also observed. The results of the present investigation indicated that the protective potential of deprenyl was probably due to the increase of the activity of the free radical scavenging enzymes or to a counteraction of free radicals by its antioxidant nature or to a strengthening of neuronal membrane by its membrane-stabilizing action. Histopathological observations also confirmed the protective effect of deprenyl against the age-related aberrations in rat cerebellum. These data on the effect of deprenyl on parameters of normal aging provides new additional information concerning the anti-aging potential of deprenyl.
抗氧化剂是自由基清除剂，可保护生物机体免受组织的氧化损伤。实验证据表明，氧自由基是衰老的重要致病因子，本研究旨在评估丙炔苯丙胺对雄性 Wistar 大鼠小脑抗氧化防御的年龄相关影响。三个年龄组（6、12 和 18 个月大）的实验大鼠每天腹膜内注射丙炔苯丙胺（2mg/kg 体重，持续 15 天），并测定血浆中诊断标志物酶（丙氨酸氨基转移酶、天冬氨酸氨基转移酶、乳酸脱氢酶和肌酸磷酸激酶）的水平、脂质过氧化物、还原型谷胱甘肽以及小脑组织中谷胱甘肽依赖的抗氧化酶（谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶）和抗过氧化物酶（过氧化氢酶和超氧化物歧化酶）的活性。丙炔苯丙胺（每天腹膜内注射 2mg/kg 体重，持续 15 天）显著（p < 0.05）减弱了实验动物血浆中诊断标志物酶水平的年龄相关改变。丙炔苯丙胺还通过在一定程度上抑制脂质过氧化来发挥抗氧化作用。还观察到还原型谷胱甘肽水平和谷胱甘肽依赖的抗氧化酶和抗过氧化物酶的活性适度升高。本研究结果表明，丙炔苯丙胺的保护潜力可能是由于自由基清除酶的活性增加，或者由于其抗氧化性质对自由基的拮抗作用，或者由于其膜稳定作用增强神经元膜。组织病理学观察也证实了丙炔苯丙胺对大鼠小脑与年龄相关的异常的保护作用。这些关于丙炔苯丙胺对正常衰老参数的影响的数据提供了有关丙炔苯丙胺抗衰老潜力的新的附加信息。
