Faraj B A, Camp V M, Kutner M
Department of Radiology, Emory University School of Medicine, Atlanta, GA 30322.
Alcohol Clin Exp Res. 1991 Feb;15(1):86-9. doi: 10.1111/j.1530-0277.1991.tb00522.x.
The main objective of this study was to determine whether the activation of dopaminergic pathways, through adrenal-caudate transplantation, stimulated the production of dopamine and salsolinol in cerebrospinal fluid (CSF) of patients with Parkinson's disease. Dopamine sulfate and salsolinol sulfate in CSF specimens were measured by radioenzymatic technique. The results of this study demonstrated that the replacement of degenerative nigrostriatal neurons with new dopamine-producing cells by adrenal brain transplants in patients with Parkinson's disease resulted in significant increase (p less than 0.05) in CSF levels of free dopamine, dopamine sulfate, free salsolinol, and salsolinol sulfate as compared with preoperative levels. Moreover, the oral administration of L-dopa to these transplanted patients caused substantial (p less than 0.001) elevation in CSF levels of free dopamine (before L-dopa, 146 +/- 57 pg/ml; after L-dopa, 575 +/- 207 pg/ml), dopamine sulfate (before L-dopa, 1966 +/- 945 pg/ml; after L-dopa, 41679 +/- 29326 pg/ml), free salsolinol (before L-dopa, 43 +/- 29 pg/ml; after L-dopa, 186 +/- 90 pg/ml), and salsolinol sulfate (before L-dopa, 405 +/- 477 pg/ml; after L-dopa, 2908 +/- 2572 pg/ml), respectively.
本研究的主要目的是确定通过肾上腺 - 尾状核移植激活多巴胺能通路是否能刺激帕金森病患者脑脊液（CSF）中多巴胺和salsolinol的产生。采用放射酶法测定脑脊液标本中的硫酸多巴胺和硫酸salsolinol。本研究结果表明，帕金森病患者通过肾上腺脑移植用新的产生多巴胺的细胞替代退化的黑质纹状体神经元后，与术前水平相比，脑脊液中游离多巴胺、硫酸多巴胺、游离salsolinol和硫酸salsolinol的水平显著升高（p < 0.05）。此外，对这些移植患者口服左旋多巴导致脑脊液中游离多巴胺（左旋多巴给药前，146 ± 57 pg/ml；左旋多巴给药后，575 ± 207 pg/ml）、硫酸多巴胺（左旋多巴给药前，1966 ± 945 pg/ml；左旋多巴给药后，41679 ± 29326 pg/ml）、游离salsolinol（左旋多巴给药前，43 ± 29 pg/ml；左旋多巴给药后，186 ± 90 pg/ml）和硫酸salsolinol（左旋多巴给药前，405 ± 477 pg/ml；左旋多巴给药后，2908 ± 2572 pg/ml）的水平大幅升高（p < 0.001）。
