未整合的病毒DNA由病毒DNA聚合酶在禽肉瘤病毒转化的鸭细胞的细胞质中合成。
Unintegrated viral DNA is synthesized in the cytoplasm of avian sarcoma virus-transformed duck cells by viral DNA polymerase.
作者信息
Varmus H E, Shank P R
出版信息
J Virol. 1976 May;18(2):567-73. doi: 10.1128/JVI.18.2.567-573.1976.
Abstract
We have examined the location, structure, and mechanism of synthesis of unintegrated viral DNA present in fully transformed cultures of avian sarcoma virus-infected duck cells. De novo synthesis of the unintegrated forms several weeks after the initial infection was documented by labeling unintegrated DNA in both strands with 5-bromodeoxyuridine. The unintegrated DNA is synthesized in, and probably confined to, the cytoplasm, and it consists of duplexes of short "plus" strands (ca. 0.5 X 10(6) to 1.0 X 10(6) daltons) and "minus" strands the length of a subunit of the viral genome (ca. 2.5 X 10(6) to 3.0 X 10(6) daltons). The structure of the duplex and the mode of incorporation of density label support the hypothesis that the unintegrated DNA is synthesized from an RNA templated by virus-coded DNA polymerase.
摘要
我们已经研究了禽肉瘤病毒感染的鸭细胞完全转化培养物中未整合病毒DNA的位置、结构和合成机制。通过用5-溴脱氧尿苷标记两条链中的未整合DNA，记录了初始感染数周后未整合形式的从头合成。未整合DNA在细胞质中合成，并且可能局限于细胞质，它由短“正”链（约0.5×10⁶至1.0×10⁶道尔顿）和病毒基因组一个亚基长度的“负”链（约2.5×10⁶至3.0×10⁶道尔顿）的双链体组成。双链体的结构和密度标记的掺入模式支持这样的假说，即未整合DNA是由病毒编码的DNA聚合酶以RNA为模板合成的。
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