Schlein R A, Kudlick E M, Reindorf C A, Gregory J, Royal G C
Howard University, Washington, D.C.
Am J Orthod Dentofacial Orthop. 1991 May;99(5):466-72. doi: 10.1016/S0889-5406(05)81580-X.
This study was made to determine whether and to what extent bacteremia occurred after toothbrushing in patients undergoing orthodontic treatment with fixed appliances. Twenty patients were selected, all with negative history of heart or hematologic disorders. These patients had not taken antibiotics or had a history of a cold in the previous 30 days. Blood samples of 20 ml were drawn before and 5 minutes after brushing. The immune status of the patients was tested by measurement of isohemagglutins and immunoglobulin levels. Blood samples were incubated in paired culture bottles containing trypticase soy broth (TSB) with an agar paddle and Columbia broth. All samples taken before brushing were negative for bacteria. Five of the 20 patients (25% of the sample) had positive blood tests after brushing. Both anaerobic and aerobic bacteria were identified from the blood samples. Those patients who were found to have a bacteremia did not display poor oral hygiene.
本研究旨在确定接受固定矫治器正畸治疗的患者在刷牙后是否会发生菌血症以及菌血症的发生程度。选取了20名患者，他们均无心脏或血液系统疾病史。这些患者在过去30天内未服用过抗生素，也没有感冒史。在刷牙前和刷牙后5分钟采集20毫升血液样本。通过检测同种血凝素和免疫球蛋白水平来测试患者的免疫状态。将血液样本在含有胰蛋白酶大豆肉汤（TSB）和琼脂搅拌棒的配对培养瓶以及哥伦比亚肉汤中培养。所有刷牙前采集的样本细菌检测均为阴性。20名患者中有5名（占样本的25%）在刷牙后血液检测呈阳性。从血液样本中鉴定出了厌氧菌和好氧菌。那些被发现有菌血症的患者口腔卫生状况并不差。
