Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2011 Feb;61(Pt 2):356-361. doi: 10.1099/ijs.0.022665-0. Epub 2010 Mar 19.
In the course of a longitudinal study elucidating the dynamics of Arcobacter populations in pigs, 16 isolates of Gram-reaction-negative, rod-shaped, slightly curved, non-spore-forming bacteria were grouped by amplified fragment length polymorphism analysis into a distinct phenon within the genus Arcobacter. Fragments were generated for all isolates in a genus-specific PCR assay, but no amplicon was obtained in a species-specific multiplex-PCR test. Numerical analysis of the whole-cell protein profiles also showed that all isolates clustered in a single group that was distinct from related members of the genus Arcobacter. DNA-DNA hybridizations between two representative strains, designated 64(T) and 122, of the isolates obtained exhibited a mean DNA-DNA relatedness of 72 %. DNA-DNA hybridizations between strains 64(T) and 122 and reference strains of other animal-related bacteria of the genus Arcobacter revealed binding values of 47 % or less. The DNA G+C contents of the two representative strains were 28.5 and 28.4 mol%, respectively, and analysis of three marker genes identified Arcobacter cryaerophilus, A. thereius, A. cibarius and A. skirrowii as their closest phylogenetic neighbours. Strains 64(T) and 122 could be distinguished from other members of the genus Arcobacter by means of biochemical tests for catalase and urease activities, nitrate reduction, indoxyl acetate hydrolysis, lack of growth at 37 °C, growth in 2 % (w/v) NaCl, growth on 0.1 % sodium deoxycholate and non-supplemented Campylobacter charcoal-deoxycholate base medium and resistance to cephalothin (32 mg l(-1)) and cefoperazone (64 mg l(-1)). Additionally, a PCR assay was developed for the detection and identification of strains 64(T) and 122, which represent a novel species of the genus Arcobacter, for which the name Arcobacter trophiarum sp. nov. is proposed. The type strain is strain 64(T) (=LMG 25534(T) =CCUG 59229(T)).
在一项阐明弧菌属种群动态的纵向研究过程中，通过扩增片段长度多态性分析，将 16 株革兰氏反应阴性、杆状、略弯曲、非孢子形成的细菌分为弧菌属内的一个独特表型。所有分离株均在属特异性 PCR 检测中生成片段，但在种特异性多重 PCR 检测中未获得扩增子。全细胞蛋白图谱的数值分析也表明，所有分离株均聚集在一个与弧菌属相关成员明显不同的单一群体中。两个代表性菌株（命名为 64(T)和 122）的 DNA-DNA 杂交显示，它们的平均 DNA-DNA 相关性为 72%。菌株 64(T)和 122 与其他动物相关弧菌属参考菌株的 DNA-DNA 杂交显示结合值小于 47%。两个代表性菌株的 DNA G+C 含量分别为 28.5%和 28.4mol%，对三个标记基因的分析表明，嗜冷弧菌、A. thereius、A. cibarius 和 A. skirrowii 是它们最接近的系统发育近邻。通过对过氧化氢酶和脲酶活性、硝酸盐还原、吲哚乙酸酯水解、37°C 下生长、2%（w/v）NaCl 中生长、0.1%脱氧胆酸钠和无补充的弯曲菌属-脱硫胆酸盐基础培养基中的生长以及头孢噻吩（32mg l(-1))和头孢哌酮（64mg l(-1))的耐药性等生化试验，可将菌株 64(T)和 122 与弧菌属的其他成员区分开来。此外，还开发了一种用于检测和鉴定菌株 64(T)和 122 的 PCR 检测方法，它们代表弧菌属的一个新种，拟命名为 Arcobacter trophiarum sp. nov.。模式菌株为菌株 64(T)（=LMG 25534(T)=CCUG 59229(T)）。
