Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146, USA.
Curr Drug Metab. 2010 Jan;11(1):1-3. doi: 10.2174/138920010791110908.
The field of drug metabolism has changed dramatically in the past generation. Two of the driving forces are the advances in analytical chemistry and our understanding of the biological systems. With regard to the former, the advances in liquid chromatography-mass spectrometry (LC-MS) are extremely impressive, and the speed of analysis has been increased even more with the recent developments in ultraperformance LC (UPLC). NMR analysis on a sub-microgram scale is not unusual, and the 2-dimensional methods are also very impressive. The biology underlying gene regulation is highly developed, and the recombinant methods have greatly facilitated progress in the field. Today it is common to design discovery and development efforts focused on critical human phenomena from the very start, with animal studies supporting the efficacy and safety efforts.
在过去的几十年里，药物代谢领域发生了巨大的变化。其中两个推动因素是分析化学的进步和我们对生物系统的理解。就前者而言，液相色谱-质谱联用（LC-MS）的进步令人印象深刻，而最近超高效液相色谱（UPLC）的发展更是提高了分析速度。纳升级别 NMR 分析已很常见，二维方法也非常出色。基因调控的生物学基础非常发达，重组方法极大地促进了该领域的发展。如今，从一开始就针对关键的人类现象设计发现和开发工作是很常见的，动物研究支持了疗效和安全性方面的努力。
