Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakhon Pathom, 73170, Thailand.
Mar Biotechnol (NY). 2011 Apr;13(2):163-9. doi: 10.1007/s10126-010-9276-0. Epub 2010 Mar 24.
Ovarian maturation in crustacean is under the control of gonad-inhibiting hormone (GIH); a neuropeptide secreted from X-organ sinus gland complex in eyestalks. Unilateral eyestalk ablation that partially destroys GIH source is therefore a general practice in Penaeus monodon hatchery to induce ovarian maturation and spawning. Our previous report showed that silencing of GIH expression by GIH-specific double-stranded RNA (GIH-dsRNA) resulted in an increased expression level of vitellogenin in P. monodon, thus suggesting that GIH-dsRNA could be an alternative method to induce ovarian maturation in female P. monodon broodstock. In this study, we further demonstrated that a single injection of GIH-dsRNA into previtellogenic female P. monodon at the concentration of 3 µg GIH-dsRNA per gram body weight of shrimp was able to inhibit GIH expression for a minimum of 30 days. This dsRNA-mediated GIH silencing led to ovarian maturation and eventual spawning in both domesticated and wild female broodstock, particularly with a comparable effect to eyestalk ablation in wild shrimp. This is the first report that demonstrates a potential strategy to induce ovarian maturation in female P. monodon broodstock by GIH-dsRNA and thus provides a possible substitute for the cruel and detrimental eyestalk ablation practice.
甲壳动物的卵巢成熟受性腺抑制激素（GIH）控制；这种神经肽由眼柄中的 X 器官-窦腺复合体分泌。因此，在斑节对虾养殖场中，单侧眼柄切除（部分破坏 GIH 来源）是诱导卵巢成熟和产卵的常用方法。我们之前的报告表明，通过 GIH 特异性双链 RNA（GIH-dsRNA）沉默 GIH 表达会导致斑节对虾中卵黄蛋白原的表达水平增加，因此表明 GIH-dsRNA 可能是诱导雌性斑节对虾亲虾卵巢成熟的替代方法。在这项研究中，我们进一步证明，在预卵期雌性斑节对虾中单次注射 3µg GIH-dsRNA/克虾体重的 GIH-dsRNA，至少可以抑制 GIH 表达 30 天。这种 dsRNA 介导的 GIH 沉默导致卵巢成熟并最终产卵，无论是在驯化还是野生的雌性亲虾中，效果与野生虾的眼柄切除相当。这是首次报道表明，通过 GIH-dsRNA 可以诱导雌性斑节对虾亲虾卵巢成熟的潜在策略，从而为残酷和有害的眼柄切除实践提供了可能的替代方法。
