Ferdous Md Akib, Islam Sk Injamamul, Habib Nasim, Almehmadi Mazen, Allahyani Mamdouh, Alsaiari Ahad Amer, Shafie Alaa
Department of Fisheries and Marine Bioscience, Faculty of Biological Science and Technology, Jashore University of Science and Technology, Jashore 7408, Bangladesh.
The International Graduate Program of Veterinary Science and Technology (VST), Department of Veterinary Microbiology, Faculty of Veterinary Science and Technology, Chulalongkorn University, Bangkok 10330, Thailand.
Microorganisms. 2022 Oct 12;10(10):2012. doi: 10.3390/microorganisms10102012.
Scientists have discovered many ways to treat bacteria, viruses, and parasites in aquaculture; however, there is still an impossibility in finding a permanent solution for all types of diseases. In that case, the CRISPR-Cas genome-editing technique can be the potential solution to preventing diseases for aquaculture sustainability. CRISPR-Cas is cheaper, easier, and more precise than the other existing genome-editing technologies and can be used as a new disease treatment tool to solve the far-reaching challenges in aquaculture. This technique may now be employed in novel ways, such as modifying a single nucleotide base or tagging a location in the DNA with a fluorescent protein. This review paper provides an informative discussion on adopting CRISPR technology in aquaculture disease management. Starting with the basic knowledge of CRISPR technology and phages, this study highlights the development of RNA-guided immunity to combat the protozoan group and nervous necrosis virus (NNV) in marine finfish. Additionally, we highlight the immunological application of CRISPR-Cas against bacterial diseases in channel catfish and the white spot syndrome virus (WSSV) in shrimp. In addition, the review summarizes a synthesis of bioinformatics tools used for CRISPR-Cas sgRNA design, and acceptable solutions are discussed, considering the limitations.
科学家们已经发现了许多治疗水产养殖中细菌、病毒和寄生虫的方法；然而，要找到针对所有类型疾病的永久解决方案仍然是不可能的。在这种情况下，CRISPR-Cas基因组编辑技术可能是实现水产养殖可持续性疾病预防的潜在解决方案。CRISPR-Cas比其他现有的基因组编辑技术更便宜、更简便且更精确，可作为一种新的疾病治疗工具来应对水产养殖中深远的挑战。现在可以以新颖的方式应用这项技术，例如修改单个核苷酸碱基或用荧光蛋白标记DNA中的某个位置。这篇综述文章就水产养殖疾病管理中采用CRISPR技术进行了有益的讨论。本研究从CRISPR技术和噬菌体的基础知识入手，重点介绍了对抗海洋硬骨鱼中原生动物群和神经坏死病毒（NNV）的RNA引导免疫的发展。此外，我们重点介绍了CRISPR-Cas在对抗斑点叉尾鮰细菌疾病和虾类白斑综合征病毒（WSSV）方面的免疫学应用。此外，该综述总结了用于CRISPR-Cas sgRNA设计的生物信息学工具的综合情况，并考虑到局限性讨论了可接受的解决方案。
