South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Molecules. 2010 Jan 29;15(2):680-8. doi: 10.3390/molecules15020680.
Harvested longan (Dimocarpus longan Lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. Ochratoxin A (OTA) is a kind of mycotoxin produced by a number of fungi. In this study, OTA was extracted from longan fruit pulp by 80% methanol and then loaded on C-18 solid-phase extraction columns. The extract solution was then analyzed by high-performance liquid chromatography - fluorescence detection (HPLC-FD) and an electron spray ionization-mass spectrometry (ESI-MS), respectively. The HPLC-FD analysis showed that a compound similar to OTA might exist in longan fruit pulp, but further analysis by the ESI-MS method demonstrated that OTA was not present in the longan pulp, indicating that the presence of OTA in longan fruit pulp detected by the HPLC-FD analysis needed to be confirmed by the ESI-MS method.
采后的龙眼（Dimocarpus longan Lour.）果实易受细菌和真菌感染引起的腐烂。赭曲霉毒素 A（OTA）是由一些真菌产生的一种霉菌毒素。在本研究中，OTA 用 80%甲醇从龙眼果肉中提取，然后加载到 C-18 固相萃取柱上。提取液分别通过高效液相色谱 - 荧光检测（HPLC-FD）和电喷雾电离 - 质谱（ESI-MS）进行分析。HPLC-FD 分析表明，龙眼果肉中可能存在类似于 OTA 的化合物，但 ESI-MS 方法的进一步分析表明，OTA 并不存在于龙眼果肉中，这表明 HPLC-FD 分析检测到的龙眼果肉中 OTA 的存在需要通过 ESI-MS 方法来确认。
