Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China.
Molecules. 2010 Jul 12;15(7):4890-7. doi: 10.3390/molecules15074890.
A new high-performance liquid chromatographic method with photodiode array detection was established for the determination of linalool in the plant Michelia alba. Linalool was extracted from the plant sample with the aid of ultrasound, and was analyzed on a Waters RP C(18) column (4.6 x 150 mm, 5 microm) using an acetonitrile and water (55:45, v/v) mobile phase at a flow rate of 1.0 mL/min. The column temperature was set at 25 degrees C, and the detection wavelength was 210 nm. The linear range of the method was 5-200 microg/mL with a correlation coefficient of 0.9975. The recovery was 92-112%, and the relative standard deviation was 1.85% (n = 9). The present method has been used to study the distribution of linalool in the plant Michelia alba. The plant samples include flowers, leaves and tender twigs. Furthermore, leaves included samples in their tender, grown-up and fallen phases, and flowers included samples in their juvenile, middle and whitening phases. The concentrations of linalool in different parts of the plant were 0.21-0.65%, 1.63-4.89% and 0.43% for leaves, flowers and tender twigs, respectively. The results showed that all the plant materials contained relative high concentration of linalool, and juvenile phase flowers contained the highest concentration of linalool. Notably, the fallen leaves also contained high concentrations of linalool, which could be a potential resource of this compound. The results obtained are very helpful for the potential full utilization of this plant.
建立了一种新的高效液相色谱法，结合光电二极管阵列检测，用于测定Michelia alba 植物中的芳樟醇。在超声辅助下，从植物样品中提取芳樟醇，在 Waters RP C(18)柱（4.6 x 150mm，5μm）上用乙腈和水（55:45，v/v）作为流动相进行分析，流速为 1.0mL/min。柱温设定为 25°C，检测波长为 210nm。该方法的线性范围为 5-200μg/mL，相关系数为 0.9975。回收率为 92-112%，相对标准偏差为 1.85%（n=9）。本方法已用于研究芳樟醇在 Michelia alba 植物中的分布。植物样品包括花、叶和嫩枝。此外，叶包括嫩、成熟和落叶期的样本，花包括幼、中、白化期的样本。植物不同部位芳樟醇的浓度分别为 0.21-0.65%、1.63-4.89%和 0.43%，叶、花和嫩枝中。结果表明，所有植物材料均含有相对较高浓度的芳樟醇，幼期花中芳樟醇含量最高。值得注意的是，落叶中也含有较高浓度的芳樟醇，可能是该化合物的潜在来源。研究结果对该植物的潜在充分利用非常有帮助。
