Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, South Korea.
Arch Virol. 2011 Mar;156(3):511-6. doi: 10.1007/s00705-010-0865-z. Epub 2010 Dec 4.
Two human G12 rotaviruses, CAU 195 and CAU 214, were isolated from South Korea using cell culture and characterized on the basis of sequence divergence in the VP7, VP4, and NSP4 genes. Phylogenetic analysis of the VP7 gene sequences indicated that these strains clustered into lineage III and were most closely related to G12 rotaviruses isolated in the United States. The VP4 and NSP4 gene sequences showed that two strains belonged to the P[6]-Ia lineage and genotype [B]. This finding provides information that can be used to evaluate G12 strains and aid in the development of effective vaccines in the future.
从韩国分离到两株人源 G12 轮状病毒 CAU195 和 CAU214，并用细胞培养法进行分离，并基于 VP7、VP4 和 NSP4 基因的序列差异进行了特征描述。VP7 基因序列的系统进化分析表明，这些毒株聚类于 III 群，与美国分离到的 G12 轮状病毒最为密切相关。VP4 和 NSP4 基因序列表明，这两个毒株属于 P[6]-Ia 谱系和基因型 [B]。这一发现提供了可以用于评估 G12 株的信息，并有助于未来开发有效的疫苗。
