Senter L H, Sanson D R, Corley D G, Tempesta M S, Rottinghaus A A, Rottinghaus G E
Ethicon Research Foundation, Ethicon, Inc., Somerville, NJ 08876-0151.
Mycopathologia. 1991 Feb;113(2):127-31. doi: 10.1007/BF00442425.
Twenty-six trichothecene mycotoxins produced by Fusarium sporotrichioides (MC-72083) and Fusarium sambucinum were screened for relative cytotoxicity in cultured baby hamster kidney (BHK-21) cells. The relative cytotoxicity was measured as LC100. The most cytotoxic trichothecenes were T-2 toxin (5 ng/ml) and the recently isolated 4-propanoyl HT-2 (5 ng/ml) and 3'-hydroxy T-2 toxin (5 ng/ml). T-2 tetraol (1 x 10(4) ng/ml), 8-beta-hydroxytrichothecene (1 x 10(4) ng/ml), sporotrichiol (2 x 10(4) ng/ml), 8-oxodiacetoxyscirpenol (6 x 10(4) ng/ml) and 8-acetyl T-2 tetraol (1 x 10(5) ng/ml) were the least toxic of the regular trichothecenes. None of the modified trichothecenes or the apotrichothecene were very cytotoxic: 8-beta-hydroxysambucoin (2 x 10(3) ng/ml), FS-1 (5 x 10(3) ng/ml), 8-alpha-hydroxysambucoin (8 x 10(4) ng/ml) and trichotriol (1 x 10(5) ng/ml). The modified trichothecenes, FS-2 and FS-3, were not toxic even at 1 x 10(5) ng/ml. The baby hamster kidney cell bioassay proved to be a very sensitive and reproducible means of screening new trichothecene mycotoxins for relative cytotoxicity.
对由拟枝孢镰刀菌（MC - 72083）和接骨木镰刀菌产生的26种单端孢霉烯族霉菌毒素，在培养的幼仓鼠肾（BHK - 21）细胞中进行了相对细胞毒性筛选。相对细胞毒性以LC100来衡量。细胞毒性最强的单端孢霉烯族毒素是T - 2毒素（5纳克/毫升）以及最近分离出的4 - 丙酰基HT - 2（5纳克/毫升）和3'-羟基T - 2毒素（5纳克/毫升）。T - 2四醇（1×10⁴纳克/毫升）、8 - β - 羟基单端孢霉烯族毒素（1×10⁴纳克/毫升）、拟枝孢镰刀菌素（2×10⁴纳克/毫升）、8 - 氧代二乙酰氧基司替戊醇（6×10⁴纳克/毫升）和8 - 乙酰基T - 2四醇（1×10⁵纳克/毫升）是常规单端孢霉烯族毒素中毒性最小的。修饰的单端孢霉烯族毒素或去端孢霉烯均无很强的细胞毒性：8 - β - 羟基接骨木镰孢菌素（2×10³纳克/毫升）、FS - 1（5×10³纳克/毫升）、8 - α - 羟基接骨木镰孢菌素（8×10⁴纳克/毫升）和单端孢三醇（1×10⁵纳克/毫升）。修饰的单端孢霉烯族毒素FS - 2和FS - 3即使在1×10⁵纳克/毫升时也没有毒性。幼仓鼠肾细胞生物测定法被证明是一种非常灵敏且可重复的方法，用于筛选新的单端孢霉烯族霉菌毒素的相对细胞毒性。
