黑骨藤的化学成分及其细胞毒性活性。
Chemical constituents of Periploca forrestii and their cytotoxicity activity.
作者信息
Wang Jin, Wang Cuifang, Chen Jinxiong, Du Jiang, Qiu Dewen, Qiu Minghua
机构信息
The State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sinences, Kunming 650204, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Dec;34(24):3214-6.
OBJECTIVE
To investigate the chemical constituents of the roots of Periploca forrestii and evaluate their cytotoxicity activities.
METHOD
Silica gel column chromatography was employed for the isolation and purification of chemical constituents. The structures were identified on the basis of spectral data and the cytotoxicities of compounds 2-4 were investigated by several tumors cell lines including blood tumor (HL-60, CCRT-CEM), prostate tumor (PC-3, DU-145) and Melanoma (UACC-62).
RESULT
Four compounds were isolated and identified as follows, lupeol-20(29)-en-3-nonadecanoate (1), peroiforoside I (2), 3beta,5beta,14beta-3OH-8beta-H-car-20(22)-enolide (3), perplocin (4).
CONCLUSION
Compound 1 is a new lupane triterpene fatty acid ester. Compounds 2-4 showed notable cytotoxicity against all tumor lines.
目的
研究大理白前根的化学成分并评估其细胞毒性活性。
方法
采用硅胶柱色谱法分离纯化化学成分。根据光谱数据鉴定结构，并通过几种肿瘤细胞系研究化合物2 - 4的细胞毒性，包括血液肿瘤（HL - 60、CCRT - CEM）、前列腺肿瘤（PC - 3、DU - 145）和黑色素瘤（UACC - 62）。
结果
分离鉴定出4个化合物，分别为羽扇豆醇-20(29)-烯-3-十九烷酸酯（1）、大理白前苷I（2）、3β,5β,14β-3-羟基-8β-H-卡尔-20(22)-烯醇内酯（3）、白前毒苷（4）。
结论
化合物1是一种新的羽扇烷型三萜脂肪酸酯。化合物2 - 4对所有肿瘤细胞系均显示出显著的细胞毒性。
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