Mogensen S C
Infect Immun. 1978 Jan;19(1):46-50. doi: 10.1128/iai.19.1.46-50.1978.
An age-dependent increase in the resistance of BALB/c mice to induction of focal necrotic hepatitis by herpes simplex virus type 2 was demonstrated. In 3-week-old mice inoculated intraperitoneally with virus, numerous necrotic foci developed in the liver. As the mice matured, the number of lesions declined until the age of 8 weeks, when no further increase in resistance appeared. Corresponding to this, the virus titers of livers and spleens of 3-week-old mice were higher than in 8-week-old animals throughout the infection, and the infection was apparently terminated in these organs of the adult mice by day 5. In vitro infection of peritoneal macrophages from 3-week-old and 8-week-old mice showed that this age-related resistance was concomitant with an increased restriction of virus replication in peritoneal macrophages from adult mice. Since, furthermore, the resistance of adult mice could be abolished by intravenous inoculation of the macrophage-toxic agent silica before infection, and since adoptive transfer of 2 X 10(6) syngeneic macrophages from adult mice to young ones conferred to the latter a resistance comparable to that of the adult mice, it is concluded that macrophage maturation is responsible for the age-dependent resistance seen in this infection.
已证实BALB/c小鼠对2型单纯疱疹病毒诱导局灶性坏死性肝炎的抵抗力存在年龄依赖性增加。在3周龄小鼠腹腔接种病毒后，肝脏出现大量坏死灶。随着小鼠成熟，病变数量减少，直至8周龄时，抵抗力不再进一步增加。与此相应，在整个感染过程中，3周龄小鼠肝脏和脾脏的病毒滴度高于8周龄动物，且成年小鼠这些器官中的感染在第5天明显终止。对3周龄和8周龄小鼠腹腔巨噬细胞进行体外感染表明，这种与年龄相关的抵抗力与成年小鼠腹腔巨噬细胞中病毒复制受限增加有关。此外，由于成年小鼠的抵抗力可在感染前通过静脉注射巨噬细胞毒性剂二氧化硅而消除，且将2×10⁶个成年小鼠同基因巨噬细胞过继转移给幼鼠可使幼鼠获得与成年小鼠相当的抵抗力，因此得出结论，巨噬细胞成熟是这种感染中所见年龄依赖性抵抗力的原因。
