Lopez C, Dudas G
Infect Immun. 1979 Feb;23(2):432-7. doi: 10.1128/iai.23.2.432-437.1979.
Studies were carried out to determine whether the in vitro capacity of adherent peritoneal cells to replicate herpes simplex virus type 1 (HSV-1) might correlate with the in vivo susceptibility of mice genetically resistant, moderately susceptible, or very susceptible to HSV-1 infection. Unstimulated and proteose peptone-stimulated monolayers restricted viral replication when infected immediately, but replicated HSV-1 when infected after 3 to 7 days of culture. Macrophages from resistant C57Bl/6 mice restricted HSV-1 replication significantly better than cells from susceptible mice. This function did not segregate with resistance, since macrophages from resistant F1 mice failed to restrict HSV-1 replication. Induction of peritoneal exudate cells with thioglycolate yielded cells capable of replicating HSV-1 when infected immediately after plating and after 4 days of culture.
开展了多项研究，以确定黏附性腹膜细胞在体外复制1型单纯疱疹病毒（HSV-1）的能力是否与对HSV-1感染具有遗传抗性、中度易感性或高度易感性的小鼠的体内易感性相关。未刺激的和经蛋白胨刺激的单层细胞在立即感染时会限制病毒复制，但在培养3至7天后感染时会复制HSV-1。来自抗性C57Bl/6小鼠的巨噬细胞比来自易感小鼠的细胞能更有效地限制HSV-1复制。由于来自抗性F1小鼠的巨噬细胞无法限制HSV-1复制，所以这种功能与抗性不相关。用巯基乙酸盐诱导腹膜渗出细胞，产生的细胞在接种后立即感染以及培养4天后感染时能够复制HSV-1。
