Loesch A, Tomlinson A, Burnstock G
Department of Anatomy and Developmental Biology, University College London, UK.
Anat Embryol (Berl). 1991;183(2):129-34. doi: 10.1007/BF00174393.
The localization of arginine-vasopressin in the endothelial cells of rat pulmonary artery was investigated by immunocytochemistry at the light and electron microscopic levels. The immunogold silver staining method was used for light microscopy of sheets of endothelium, removed from the artery, and the pre-embedding peroxidase-antiperoxidase technique was used for electron microscopy of cross sections of the artery. With both of the methods used, numerous vasopressin-positive endothelial cells were observed. None of the subendothelial elements showed labelling for vasopressin. The results are discussed in terms of the involvement of the endothelium in local control of the pulmonary circulation.
采用免疫细胞化学方法，在光镜和电镜水平上研究了大鼠肺动脉内皮细胞中精氨酸加压素的定位。免疫金银染色法用于对从动脉分离出的内皮细胞片进行光镜观察，而包埋前过氧化物酶-抗过氧化物酶技术则用于对动脉横断面进行电镜观察。使用这两种方法均观察到大量加压素阳性内皮细胞。内皮下层的所有成分均未显示加压素标记。根据内皮在肺循环局部控制中的作用对结果进行了讨论。
