Nakamura S, Naruse M, Naruse K, Demura H, Uemura H
Biological Laboratory, Kanagawa Dental College, Yokosuka, Japan.
Histochemistry. 1990;94(5):475-7. doi: 10.1007/BF00272609.
To investigate the intracellular localization of endothelin in cultured endothelial cells, an immunocytochemical study was carried out by the post-embedding protein A-gold technique with endothelin-specific antiserum. Gold particles were seen on the rough endoplasmic reticulum, the Golgi cisternae, the Golgi vesicles, small vesicles beneath the cell membrane, and the lysosomes. By contrast, no secretory granules were observed. These results suggest that endothelin is secreted by a constitutive pathway and that the lysosome may play an important role in regulating the biological activity of endothelin.
为研究内皮素在培养的内皮细胞中的细胞内定位，采用包埋后蛋白A-金技术，使用内皮素特异性抗血清进行了免疫细胞化学研究。在粗面内质网、高尔基池、高尔基小泡、细胞膜下的小泡和溶酶体上可见金颗粒。相比之下，未观察到分泌颗粒。这些结果表明，内皮素通过组成型途径分泌，溶酶体可能在调节内皮素的生物学活性中起重要作用。
