应用不同种属抗原检测人脑囊虫病患者 IgA。
IgA detection in human neurocysticercosis using different preparations of heterologous antigen.
机构信息
Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, CEP 38400-902, Uberlândia, Minas Gerais, Brazil.
出版信息
Parasitol Res. 2010 Jun;107(1):221-5. doi: 10.1007/s00436-010-1859-1. Epub 2010 Apr 13.
Neurocysticercosis (NC) is the most important neurological disease of parasitic origin in humans. IgA and IgG detection in serum from neurocysticercosis patients was tested using some antigenic preparations of total saline extract from Taenia saginata: detergent (D) and aqueous (A) phases extracted with Triton X-114 and the jacalin bound (JBF) and unbound fractions (JUF) obtained by affinity chromatography using jacalin column. Samples were obtained from 45 patients with definitive NC, who were subdivided into active-NC group and inactive-NC group; 35 patients with other parasitoses; and 30 apparently healthy individuals. Sensitivity and specificity were calculated. Specificity to detect IgA and IgG for D phase, respectively, were 89.8% and 86.9% and for IgG detection 91.3% and 76.8% when using D phase and JUF, respectively. D phase and JBF proved to be specific and efficient and could be efficiently utilized as an alternative antigen for IgA detection in NC, with comparable results with IgG.
脑囊尾蚴病（NC）是人类最重要的寄生虫源性神经疾病。使用猪带绦虫总盐水提取物的一些抗原制剂检测神经囊尾蚴病患者血清中的 IgA 和 IgG：用 Triton X-114 提取的去污剂（D）和水相（A）以及用亲和层析用 jacalin 柱获得的结合（JBF）和未结合部分（JUF）。从 45 例明确诊断为 NC 的患者中采集样本，将其分为活动型 NC 组和非活动型 NC 组；35 例其他寄生虫病患者；30 名貌似健康的个体。计算了敏感性和特异性。分别使用 D 相和 JUF 检测 D 相中 IgA 和 IgG 的特异性为 89.8%和 86.9%，而 IgG 检测的特异性为 91.3%和 76.8%。D 相和 JBF 被证明是特异且有效的，可以有效地用作 NC 中 IgA 检测的替代抗原，与 IgG 的检测结果相当。
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