Department of Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand;
Persoonia. 2008 Dec;21:77-91. doi: 10.3767/003158508X370857. Epub 2008 Sep 24.
Species of Mycosphaerella and their related anamorphs represent potentially serious foliar pathogens of Eucalyptus. The fungi treated in the present study were isolated from symptomatic Eucalyptus leaves collected in Thailand during June-October 2007. Species were initially identified based on morphological and cultural characteristics. Identifications were confirmed using comparisons of DNA sequence data of the internal transcribed spacers (ITS1, 5.8S nrDNA, ITS2) and the 28S nrDNA (LSU) regions. To help distinguish species of Pseudocercospora, the dataset was expanded by generating partial sequences of the translation elongation factor 1-alpha and actin genes. By integrating the morphological and molecular datasets, five new taxa were distinguished, namely Mycosphaerella irregulari, M. pseudomarksii, M. quasiparkii, Penidiella eucalypti and Pseudocercospora chiangmaiensis, while M. vietnamensis represents a new record for Thailand.
无性型真菌和相关无性型种属是潜在的严重威胁桉树叶片的病原菌。本研究中处理的真菌是于 2007 年 6 月至 10 月期间从泰国收集的有症状桉树叶片中分离出来的。最初根据形态学和培养特性对物种进行了鉴定。通过比较 ITS1、5.8S nrDNA、ITS2 区和 28S nrDNA（LSU）区的 DNA 序列数据，对鉴定结果进行了确认。为了帮助区分拟尾孢属的物种，通过生成翻译延伸因子 1-α和肌动蛋白基因的部分序列扩展了数据集。通过整合形态学和分子数据集，区分出了五个新的分类群，即不规则球腔菌、伪马克氏球腔菌、准帕克球腔菌、桉树短梗霉和清迈拟尾孢菌，而越南球腔菌则代表了泰国的一个新记录。
