Quaedvlieg W, Binder M, Groenewald J Z, Summerell B A, Carnegie A J, Burgess T I, Crous P W
CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands;
Royal Botanic Gardens and Domain Trust, Mrs. Macquaries Road, Sydney, NSW 2000, Australia.
Persoonia. 2014 Dec;33:1-40. doi: 10.3767/003158514X681981. Epub 2014 May 15.
The Teratosphaeriaceae represents a recently established family that includes numerous saprobic, extremophilic, human opportunistic, and plant pathogenic fungi. Partial DNA sequence data of the 28S rRNA and RPB2 genes strongly support a separation of the Mycosphaerellaceae from the Teratosphaeriaceae, and also provide support for the Extremaceae and Neodevriesiaceae, two novel families including many extremophilic fungi that occur on a diversity of substrates. In addition, a multi-locus DNA sequence dataset was generated (ITS, LSU, Btub, Act, RPB2, EF-1α and Cal) to distinguish taxa in Mycosphaerella and Teratosphaeria associated with leaf disease of Eucalyptus, leading to the introduction of 23 novel genera, five species and 48 new combinations. Species are distinguished based on a polyphasic approach, combining morphological, ecological and phylogenetic species concepts, named here as the Consolidated Species Concept (CSC). From the DNA sequence data generated, we show that each one of the five coding genes tested, reliably identify most of the species present in this dataset (except species of Pseudocercospora). The ITS gene serves as a primary barcode locus as it is easily generated and has the most extensive dataset available, while either Btub, EF-1α or RPB2 provide a useful secondary barcode locus.
球腔菌科是一个最近建立的科，包括许多腐生菌、嗜极端环境菌、人类机会致病菌和植物病原菌。28S rRNA和RPB2基因的部分DNA序列数据有力地支持了将球座菌科与球腔菌科分开，同时也为极端菌科和新德弗里斯菌科提供了支持，这两个新科包括许多生长在多种基质上的嗜极端环境真菌。此外，还生成了一个多位点DNA序列数据集（ITS、LSU、Btub、Act、RPB2、EF-1α和Cal），以区分与桉树叶病相关的球座菌属和球腔菌属中的分类单元，从而引入了23个新属、5个种和48个新组合。物种是基于多相方法来区分的，结合了形态学、生态学和系统发育物种概念，这里命名为综合物种概念（CSC）。从生成的DNA序列数据中，我们表明所测试的五个编码基因中的每一个都能可靠地识别该数据集中存在的大多数物种（假尾孢属物种除外）。ITS基因作为主要的条形码位点，因为它易于生成且有最广泛的可用数据集，而Btub、EF-1α或RPB2则提供了有用的二级条形码位点。
