Ha Yun-Sok, Kim Min-Ju, Yoon Hyung-Yoon, Kang Ho-Won, Kim Yong-June, Yun Seok-Joong, Lee Sang-Cheol, Kim Wun-Jae
Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea.
Korean J Urol. 2010 Jan;51(1):15-20. doi: 10.4111/kju.2010.51.1.15. Epub 2010 Jan 21.
S100A8 is a member of the S100 protein family containing 2EF-hand calcium-binding motifs. S100 proteins are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with various diseases and cancers. The present study aimed to evaluate whether S100A8 has prognostic value for non-muscle-invasive bladder cancer (NMIBC).
A total of 103 primary NMIBC samples obtained by transurethral resection were evaluated. mRNA levels were examined by real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis. The results were compared with clinico-pathological parameters. The Kaplan-Meier method was applied to plot the curves for progression-free survival. The multivariate Cox regression model was used to identify the independent prognostic factors for progression.
mRNA expression levels of S100A8 were significantly related to the progression of NMIBC. Kaplan-Meier estimates demonstrated significant differences in tumor progression according to the level of S100A8 expression (log-rank test, p<0.001). The multivariate Cox regression model revealed that the S100A8 mRNA expression level (hazard ratio: 12.538; 95% confidence interval: 2.245-70.023, p=0.004) was an independent predictor for disease progression of NMIBC.
Expression levels of S100A8 might be a useful prognostic marker for disease progression of NMIBC.
S100A8是S100蛋白家族的成员，含有2个EF手型钙结合基序。S100蛋白参与多种细胞过程的调控，如细胞周期进程和分化。该蛋白表达改变与多种疾病和癌症相关。本研究旨在评估S100A8对非肌层浸润性膀胱癌（NMIBC）是否具有预后价值。
共评估了103例经尿道切除术获取的原发性NMIBC样本。通过实时逆转录聚合酶链反应（RT-PCR）分析检测mRNA水平。将结果与临床病理参数进行比较。采用Kaplan-Meier法绘制无进展生存曲线。使用多变量Cox回归模型确定进展的独立预后因素。
S100A8的mRNA表达水平与NMIBC的进展显著相关。Kaplan-Meier估计显示，根据S100A8表达水平，肿瘤进展存在显著差异（对数秩检验，p<0.001）。多变量Cox回归模型显示，S100A8 mRNA表达水平（风险比：12.538；95%置信区间：2.245 - 70.023，p = 0.004）是NMIBC疾病进展的独立预测因子。
S100A8的表达水平可能是NMIBC疾病进展的有用预后标志物。
