血管紧张素转换酶基因插入/缺失多态性与埃塞俄比亚人群高血压风险的关联。

Association of angiotensin-converting enzyme gene insertion/deletion polymorphisms with risk of hypertension among the Ethiopian population.

机构信息

Department of Environmental and Occupational Health and Safety, Institute of Public Health, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.

Department of Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.

出版信息

PLoS One. 2022 Nov 10;17(11):e0276021. doi: 10.1371/journal.pone.0276021. eCollection 2022.

DOI:10.1371/journal.pone.0276021

PMID:36355860

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9648817/

Abstract

INTRODUCTION

Although the pathophysiological mechanism of hypertension is not fully elucidated yet, a large number of pieces of evidence have shown that genetic alterations in the renin-angiotensin-aldosterone system play a central role. However, the association of insertion/deletion polymorphism of the angiotensin-converting enzyme (ACE) gene with essential hypertension is controversial yet, and there is a limited number of publications among the Ethiopian population. Therefore, this study aimed to determine the association of ACE gene I/D polymorphism with the risk of hypertension among essential hypertension patients at the University of Gondar Comprehensive Specialized Hospital, Gondar, Ethiopia.

MATERIALS AND METHODS

A case-control study was conducted from October 07, 2020, to June 02, 2021, among hypertensive patients and normotensive control groups at the University of Gondar Comprehensive Specialized Hospital. A structured questionnaire was used to collect socio-demographic data and anthropometric measurements. Five milliliters of blood were drawn from each of the randomly selected 64 hypertensive and 64 normotensive participants for molecular test analysis. Genetic polymorphism of the ACE gene was identified using polymerase chain reaction (PCR) and electrophoresis. Data analysis was done using SPSS version 25.0 software. The strength of association between the genotype and hypertension was estimated through the calculation of adjusted odds ratio and 95% confidence intervals using logistic regression. P-value < 0.05 was considered statistically significant.

RESULT

The distribution of DD genotypes and D allele of the ACE gene were 48.4% and 63% in essential hypertensive patients, respectively, while it were 29.7% and 42.2% in control subjects respectively. The ACE DD genotype (p-value = 0.005) and D allele (p-value = 0.001) were more frequent among hypertensive patients as compared to controls.

CONCLUSION

The present study found that the DD genotype and D allele of the ACE gene has had a strong association with a high risk of hypertension in the study population.

摘要

简介

尽管高血压的病理生理机制尚未完全阐明，但大量证据表明肾素-血管紧张素-醛固酮系统的遗传改变起着核心作用。然而，血管紧张素转换酶（ACE）基因插入/缺失多态性与原发性高血压的相关性仍存在争议，并且在埃塞俄比亚人群中的出版物数量有限。因此，本研究旨在确定 ACE 基因 I/D 多态性与在埃塞俄比亚贡德尔大学综合专科医院的原发性高血压患者中高血压风险的相关性。

材料和方法

2020 年 10 月 7 日至 2021 年 6 月 2 日，在贡德尔大学综合专科医院，进行了一项病例对照研究，包括高血压患者和血压正常对照组。使用结构化问卷收集社会人口统计学数据和人体测量数据。从随机选择的 64 名高血压患者和 64 名血压正常对照组中抽取 5 毫升血液进行分子测试分析。使用聚合酶链反应（PCR）和电泳法鉴定 ACE 基因的遗传多态性。使用 SPSS 版本 25.0 软件进行数据分析。使用逻辑回归计算调整后的优势比和 95%置信区间来估计基因型与高血压之间的关联强度。p 值 < 0.05 被认为具有统计学意义。