Department of Pharmacology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
J Ethnopharmacol. 2010 Jul 6;130(1):85-92. doi: 10.1016/j.jep.2010.04.014. Epub 2010 Apr 24.
Achillea millefolium L. is a member of the Asteraceae family that is commonly referred to as "yarrow" and has been used in folk medicine against several disturbances including skin inflammations, spasmodic and gastrointestinal disorders, as well as hepato-biliary complaints.
In the present study, we evaluated the efficacy of a hydroalcoholic extract from the Achillea millefolium (HE) for gastroprotective properties and additional mechanism(s) involved in this activity.
Rats were treated with HE and subsequently exposed to both acute gastric lesions induced by ethanol P.A. and chronic gastric ulcers induced by 80% acetic acid. Following treatment, glutathione (GSH) levels and superoxide dismutase (SOD) activity were measured. The activity of myeloperoxidase (MPO) and histological and immunohistochemical analysis were performed in animals with acetic acid-induced gastric ulcers.
Oral administration of HE (30, 100 and 300mg/kg) inhibited ethanol-induced gastric lesions by 35, 56 and 81%, respectively. Oral treatment with HE (1 and 10mg/kg) reduced the chronic gastric ulcers induced by acetic acid by 43 and 65%, respectively, and promoted significant regeneration of the gastric mucosa after ulcer induction denoting increased cell proliferation, which was confirmed by PCNA immunohistochemistry. HE treatment prevented the reduction of GSH levels and SOD activity after acetic acid-induced gastric lesions. In addition, HE (10mg/kg) inhibited the MPO activity in acetic acid-induced gastric ulcers.
The results of the present study indicate that the antioxidant properties of HE may contribute to the gastroprotective activity of this extract.
蓍草（Achillea millefolium L.）是菊科的一种，通常被称为“蓍草”，在民间医学中被用于治疗多种疾病，包括皮肤炎症、痉挛和胃肠道疾病以及肝胆疾病。
本研究评估了蓍草（HE）水醇提取物的胃保护作用及其在该活性中涉及的其他机制。
用 HE 处理大鼠，然后暴露于乙醇 P.A. 引起的急性胃损伤和 80%乙酸引起的慢性胃溃疡。治疗后，测量谷胱甘肽 (GSH) 水平和超氧化物歧化酶 (SOD) 活性。在乙酸诱导的胃溃疡动物中进行髓过氧化物酶 (MPO) 的活性以及组织学和免疫组织化学分析。
HE（30、100 和 300mg/kg）的口服给药分别抑制了乙醇诱导的胃损伤 35%、56%和 81%。HE（1 和 10mg/kg）的口服治疗分别减少了 43%和 65%的乙酸诱导的慢性胃溃疡，并促进了溃疡诱导后胃黏膜的显著再生，这表明细胞增殖增加，PCNA 免疫组化证实了这一点。HE 处理可防止乙酸诱导的胃损伤后 GSH 水平和 SOD 活性的降低。此外，HE（10mg/kg）抑制了乙酸诱导的胃溃疡中的 MPO 活性。
本研究结果表明，HE 的抗氧化特性可能有助于该提取物的胃保护活性。
