Kuvezdić Koraljka Gjadrov, Aurer Igor, Ries Suncica, Sucić Mirna, Glamocak Mirjana Marković, Ilić Ivana, Basić-Kinda Sandra, Radman Ivo, Labar Boris
Department of Pathology and Cytology, University Hospital Center Zagreb, Zagreb, Croatia.
Coll Antropol. 2010 Mar;34(1):7-12.
Fine-needle aspiration (FNA) biopsy has become a well established technique in the diagnosis, staging, and follow-up of patients with head and neck lesions. As in lymphoma diagnostics, FNA serves as a screening method in evaluating potentially affected lymph node for open or core biopsy. According to the World Health Organization classification of lymphoid neoplasms, today it is important to recognize cell morphology and reveal its phenotype, then combine it with different genotypic information and clinical data to provide appropriate therapy. The aim of this study was to assess the efficacy of FNA and immunocytochemistry based lymphoma diagnostic in head and neck region. We conducted a retrospective study during a period of three years where cases with either FNA diagnosis or clinical suspicion of newly recognized or relapsing lymphoma were reviewed. In the study were included patients that were referred to our laboratory from hematology department, in whom head and neck lymphadenopathia was found and lymph node FNA preceded other procedures. Two hundred eighty-five aspirations from 248 patients fulfilled study criteria. Adequate specimens were diagnosed as lymphoma in 100 cases (36%), in 65 male and 35 female patients, 76 in patients with newly discovered disease and 24 in patients with prior lymphoma diagnosis. Overall sensitivity of FNA specimens in the diagnosis of head and neck lymphomas was 90%, specificity 88%, predictive value of a positive result 97%, and predictive value of negative result 61%. Based on our results FNA corroborated with immunophenotyping by immunocytochemistry can be method of choice in primary lymphoma diagnosis as a method complementary to histopathology in lymphoma diagnostics.
细针穿刺抽吸活检（FNA）已成为头颈部病变患者诊断、分期及随访的成熟技术。如同淋巴瘤诊断一样，FNA作为一种筛查方法，用于评估可能受累的淋巴结，以决定是否进行开放性或粗针活检。根据世界卫生组织淋巴样肿瘤分类，如今认识细胞形态并揭示其表型，然后将其与不同的基因型信息及临床数据相结合以提供恰当治疗非常重要。本研究的目的是评估FNA及基于免疫细胞化学的淋巴瘤诊断对头颈部区域的有效性。我们进行了一项为期三年的回顾性研究，对FNA诊断或临床怀疑为新发现或复发淋巴瘤的病例进行了复查。纳入研究的患者是从血液科转至我们实验室的，这些患者发现有头颈部淋巴结病，且在进行其他检查之前先进行了淋巴结FNA。248例患者的285次穿刺符合研究标准。100例（36%）标本被确诊为淋巴瘤，其中男性65例，女性35例，76例为新发现疾病患者，24例为先前诊断为淋巴瘤的患者。FNA标本对头颈部淋巴瘤诊断的总体敏感性为90%，特异性为88%，阳性结果预测值为97%，阴性结果预测值为61%。基于我们的结果，FNA联合免疫细胞化学免疫表型分析可作为原发性淋巴瘤诊断的首选方法，作为淋巴瘤诊断中组织病理学的补充方法。
