豚鼠气管对电场刺激的非肾上腺素能非胆碱能舒张反应部分由一氧化氮介导的证据。

Evidence that part of the NANC relaxant response of guinea-pig trachea to electrical field stimulation is mediated by nitric oxide.

Li C G, Rand M J

Department of Pharmacology, University of Melbourne, Victoria, Australia.

Br J Pharmacol. 1991 Jan;102(1):91-4. doi: 10.1111/j.1476-5381.1991.tb12137.x.

The nitric oxide (NO) synthesis inhibitors NG-monomethyl L-arginine (L-NMMA) and L-nitroarginine methyl ester (L-NAME) reduced relaxations of guinea-pig tracheal smooth muscle elicited by stimulation of intramural non-adrenergic, non-cholinergic (NANC) nerves, but D-NMMA had no effect. L-NAME was 10-30 times more potent than L-NMMA. Relaxations produced by sodium nitroprusside and vasoactive intestinal polypeptide (VIP) were not affected by L-NMMA or L-NAME. 2. The inhibitory effect of L-NMMA on NANC-mediated relaxations was partially reversed by L-arginine but was not affected by D-arginine. 3. VIP antibody and alpha-chymotrypsin abolished or greatly reduced the relaxant action of VIP and reduced relaxations elicited by stimulation of NANC nerves; the residual NANC relaxation was further reduced by L-NAME. 4. The results suggest that NO and VIP are mediators of NANC-induced relaxations of guinea-pig tracheal smooth muscle. We propose the term 'nitrergic' to describe transmission processes which are mediated by NO.

一氧化氮（NO）合成抑制剂N-甲基-L-精氨酸（L-NMMA）和L-硝基精氨酸甲酯（L-NAME）可降低刺激豚鼠气管壁非肾上腺素能、非胆碱能（NANC）神经所引起的气管平滑肌舒张，但D-NMMA无此作用。L-NAME的效力比L-NMMA强10至30倍。硝普钠和血管活性肠肽（VIP）所产生的舒张不受L-NMMA或L-NAME的影响。2. L-精氨酸可部分逆转L-NMMA对NANC介导舒张的抑制作用，但D-精氨酸无此作用。3. VIP抗体和α-糜蛋白酶可消除或大大降低VIP的舒张作用，并减少刺激NANC神经所引起的舒张；L-NAME可进一步降低残余的NANC舒张。4. 结果表明，NO和VIP是豚鼠气管平滑肌NANC诱导舒张的介质。我们提出用“氮能”一词来描述由NO介导的传递过程。

Evidence that part of the NANC relaxant response of guinea-pig trachea to electrical field stimulation is mediated by nitric oxide.