在斑马鱼中产生和检测等离子体纳米气泡。
Generation and detection of plasmonic nanobubbles in zebrafish.
机构信息
Rice University, TX 77005, USA.
出版信息
Nanotechnology. 2010 Jun 4;21(22):225102. doi: 10.1088/0957-4484/21/22/225102. Epub 2010 May 7.
Abstract
The zebrafish embryo has been evaluated as an in vivo model for plasmonic nanobubble (PNB) generation and detection at nanoscale. The embryo is easily observed and manipulated utilizing the same methodology as for application of PNBs in vitro. Injection and irradiation of gold nanoparticles with a short laser pulse resulted in generation of PNBs in zebrafish with similar parameters as for PNBs generated in water and cultured living cells. These PNBs do not result in systemic damage, thus we demonstrated an in vivo model for rapid and precise testing of plasmonic nanotechnologies.
摘要
斑马鱼胚胎已被评估为用于在纳米尺度上生成和检测等离子体纳米气泡 (PNB) 的体内模型。胚胎很容易通过与体外应用 PNB 相同的方法进行观察和操作。利用短激光脉冲将金纳米粒子注入和辐照到斑马鱼体内，会产生与在水中和培养的活细胞中产生的 PNB 具有相似参数的 PNB。这些 PNB 不会导致全身损伤，因此我们展示了一种用于快速、精确测试等离子体纳米技术的体内模型。
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