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基于 Luminex 技术的多重快速鉴定念珠菌分离株种属的方法在一项持续性念珠菌血症监测中的应用。

Utility of a Luminex-based assay for multiplexed, rapid species identification of Candida isolates from an ongoing candidemia surveillance.

机构信息

Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.

出版信息

Can J Microbiol. 2010 Apr;56(4):348-51. doi: 10.1139/w10-003.

DOI:10.1139/w10-003
PMID:20453902
Abstract

A Candida-specific Luminex-based assay with 11 probes was employed for multiplexed, rapid identification of 1182 Candida sp. isolates that were received as part of an ongoing population-based surveillance. All the Candida isolates were previously identified by a combination of methods, including phenotype and sequence analysis. Results showed that the Luminex assay was an attractive alternative to reference methods, as it is rapid, yields correct species identification, and is user friendly.

摘要

采用了一种基于 Luminex 的针对念珠菌的 11 探针多重检测方法,对作为正在进行的基于人群的监测的一部分而收到的 1182 株念珠菌进行了快速鉴定。所有的念珠菌分离株都已经过包括表型和序列分析在内的多种方法进行了鉴定。结果表明,该 Luminex 检测法是一种有吸引力的替代参考方法,因为它快速、准确地鉴定物种,而且易于使用。

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