Del Pozzo Giovanna, Mascolo Dina, Sartorius Rossella, Citro Alessandra, Barba Pasquale, D'Apice Luciana, De Berardinis Piergiuseppe
Institute of Genetics and Biophysics "Adriano Buzzati Traverso", CNR, Via P. Castellino 111, 80131 Naples, Italy.
J Biomed Biotechnol. 2010;2010:894971. doi: 10.1155/2010/894971. Epub 2010 Apr 29.
The ability of fd bacteriophage particles to trigger different arms of the immune system has been previously shown by us with particular emphasis on the ability of phages to raise CTL responses in vitro and in vivo. Here we show that fd virions in the absence of adjuvants are able to evoke a DTH reaction mediated by antigen specific CD8+ T cells. In addition, we analyzed the induction of CTL responses in mice depleted of CD4+ T cells, and we observed that short-term secondary CTL responses were induced in the absence of CD4+ T cells while induction of long-term memory CTLs required the presence of CD4+ T lymphocytes. These results examine the cellular mechanism at the basis of fd efficiency and provide new elements to further validate the use of fd particles for eliciting and monitoring antigen-specific CTLs.
我们之前已经证明了fd噬菌体颗粒激发免疫系统不同分支的能力，特别强调了噬菌体在体外和体内引发细胞毒性T淋巴细胞（CTL）反应的能力。在此我们表明，在没有佐剂的情况下，fd病毒粒子能够引发由抗原特异性CD8 + T细胞介导的迟发型超敏反应（DTH）。此外，我们分析了在CD4 + T细胞耗竭的小鼠中CTL反应的诱导情况，并且我们观察到在没有CD4 + T细胞的情况下诱导了短期二次CTL反应，而长期记忆CTL的诱导需要CD4 + T淋巴细胞的存在。这些结果研究了fd效率基础上的细胞机制，并提供了新的要素以进一步验证使用fd颗粒引发和监测抗原特异性CTL的方法。
