Laboratório de Inovações em Terapias, Ensino e Bioprodutos (LITEB), Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Pavilhão Cardoso Fontes, sala 52, Av. Brasil, 4365 Manguinhos, Rio de Janeiro, RJ 21045-360, Brazil.
Parasitol Res. 2010 Jun;107(1):239-42. doi: 10.1007/s00436-010-1871-5. Epub 2010 May 8.
Chagas disease, caused by Trypanosoma cruzi, is an important public health problem in Latin America. Disturbances in gastrointestinal motility are observed in 15-20% of patients at the chronic phase. We previously observed a decrease in intestinal motility in mice infected with Y strain from T. cruzi. Thus, we decided to test if infection with other T. cruzi strains also caused the intestinal disturbance. Male adult Swiss mice were infected intraperitoneally with CL-Brener clone (CL-B), Brazil strain (Br), or Dm28 clone (Dm) of T. cruzi. All infected mice presented a low cumulative mortality (CL-B, 17%; Br, 8%; Dm, 25%) at 35 days post infection (dpi) and their typical parasitemia curves. Br and Dm groups exhibited a maximal reduction of intestinal motility at 35 dpi (176.8 +/- 51.3 and 198.3 +/- 52.6 min, respectively), when compared with non-infected mice (90.2 +/- 19.5 min). However, CL mice presented the peak of delayed intestinal transit at 12 dpi (191.0 +/- 33.3 min), when compared with non-infected mice (105.6 +/- 26.4 min), very close to the 15 dpi for the intense alteration (310.2 +/- 67.4 min) observed with the Y strain. We clearly demonstrate a reduction in intestinal motility in mice infected with different groups of T. cruzi during the acute phase of the infection. Since Br, Dm, and CL strains presented low mortality rates in adult Swiss mice, a prospective study concerning the chronic intestinal alteration is encouraged, particularly for studies of alternative therapies.
恰加斯病是由克氏锥虫引起的，是拉丁美洲的一个重要公共卫生问题。在慢性期，15-20%的患者会出现胃肠蠕动紊乱。我们之前观察到感染 Y 株克氏锥虫的小鼠肠道蠕动减少。因此，我们决定测试其他 T. cruzi 株感染是否也会引起肠道紊乱。雄性成年瑞士小鼠经腹腔感染 CL-Brener 克隆（CL-B）、巴西株（Br）或 Dm28 克隆（Dm）的 T. cruzi。所有感染的小鼠在感染后 35 天（dpi）都表现出低累积死亡率（CL-B，17%；Br，8%；Dm，25%）和典型的寄生虫血症曲线。Br 和 Dm 组在 35 dpi 时肠道蠕动最大减少（分别为 176.8 +/- 51.3 和 198.3 +/- 52.6 min），与未感染的小鼠相比（90.2 +/- 19.5 min）。然而，CL 小鼠在 12 dpi 时表现出肠道传输延迟的峰值（191.0 +/- 33.3 min），与未感染的小鼠相比（105.6 +/- 26.4 min），非常接近 Y 株引起的强烈改变的 15 dpi（310.2 +/- 67.4 min）。我们清楚地证明了在感染的急性期中，不同组别的 T. cruzi 感染的小鼠肠道蠕动减少。由于 Br、Dm 和 CL 株在成年瑞士小鼠中的死亡率较低，鼓励对慢性肠道改变进行前瞻性研究，特别是对替代治疗的研究。
