Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark.
J Appl Microbiol. 2010 Sep;109(3):1017-26. doi: 10.1111/j.1365-2672.2010.04729.x.
To monitor seasonal variations in the microbiology of maize silage and to determine whether the risk of fungal spoilage varies during whole-year storage.
A continuous survey of 20 maize silage stacks was conducted over a period from three to 11 months after ensiling. Filamentous fungi, yeasts and lactic acid bacteria (LAB) were enumerated at five time-points, and cultivable species of filamentous fungi were identified. Significant differences in the numbers of filamentous fungi, yeast and LAB were detected. The highest numbers of fungi were five to seven and the lowest 11 months after ensiling, while the LAB decreased in numbers during the study. Filamentous fungi were isolated from all stacks at all time-points. The most abundant toxigenic mould species were Penicillium roqueforti, Penicillium paneum and Aspergillus fumigatus.
There are significant variations in the microbiology of maize silage over a whole storage season. The risk of fungal spoilage was highest 5-7 months after ensiling and lowest after 11 months.
This information is valuable in the assessment of health risks connected with spoiled maize silage and may be useful in the management of maize silage stacks, when whole-season storage is applied.
监测玉米青贮微生物学的季节性变化，并确定在全年储存过程中真菌腐败的风险是否会发生变化。
在青贮后 3 至 11 个月的时间内，对 20 个玉米青贮堆进行了连续监测。在五个时间点对丝状真菌、酵母和乳酸菌 (LAB) 进行了计数，并对可培养的丝状真菌进行了鉴定。检测到丝状真菌、酵母和 LAB 的数量存在显著差异。真菌数量最高的是青贮后 5 至 7 个月，最低的是 11 个月，而 LAB 在研究过程中数量减少。所有青贮堆在所有时间点都分离出了丝状真菌。最丰富的产毒霉菌种类为青霉属、青霉属和烟曲霉。
在整个储存季节，玉米青贮的微生物学存在显著变化。真菌腐败的风险在青贮后 5-7 个月最高，11 个月后最低。
该信息对于评估与变质玉米青贮相关的健康风险非常有价值，并且在应用全季储存时，对于管理玉米青贮堆可能会很有用。
