Li Xiaomei, Chen Fei, Wang Xuekai, Sun Lin, Guo Linna, Xiong Yi, Wang Yuan, Zhou Hongzhang, Jia Shangang, Yang Fuyu, Ni Kuikui
College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China.
Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China.
Microorganisms. 2021 Jan 28;9(2):274. doi: 10.3390/microorganisms9020274.
The objective of this study was to investigate how storage temperatures influence the bacterial community of oat silage during the ensiling process via PacBio single molecule, real-time sequencing technology (SMRT). Forage oat was ensiled at four different temperatures (5 °C, 10 °C, 15 °C, and 25 °C) and ensiling days (7, 14, 30, and 60 days). With the rise in storage temperature, the lactic acid content showed an increased trend. Acetic acid production was observed highest in silage fermented at 5 °C compared with other treatments, and was also the dominant bacterial species. and were exclusively detected in silages at 10 °C, 15 °C, and 25 °C, and dominated the fermentation after 60 days of ensiling at 10 °C and 25 °C, respectively. In addition, , , and may be related to changes in the fermentation products due to the differences in ensiling temperature. In conclusion, results of this study improve our understanding of the complicated microbial composition underlying silage fermentation at low temperatures, which might contribute to target-based regulation methods for enhancing silage quality and developing new inoculants.
本研究的目的是通过PacBio单分子实时测序技术（SMRT），研究贮藏温度如何在青贮过程中影响燕麦青贮饲料的细菌群落。将饲用燕麦在四个不同温度（5℃、10℃、15℃和25℃）和青贮天数（7天、14天、30天和60天）下进行青贮。随着贮藏温度的升高，乳酸含量呈上升趋势。与其他处理相比，在5℃下发酵的青贮饲料中乙酸产量最高，且也是优势细菌种类。[未明确的细菌名称1]和[未明确的细菌名称2]分别仅在10℃、15℃和25℃的青贮饲料中检测到，且分别在10℃和25℃青贮60天后在发酵过程中占主导地位。此外，[未明确的细菌名称3]、[未明确的细菌名称4]和[未明确的细菌名称5]可能由于青贮温度的差异而与发酵产物的变化有关。总之，本研究结果增进了我们对低温青贮发酵过程中复杂微生物组成的理解，这可能有助于制定基于目标的调控方法以提高青贮饲料质量并开发新型接种剂。
