Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, New Hampshire 03755, USA.
J Biomed Opt. 2010 Mar-Apr;15(2):026028. doi: 10.1117/1.3374040.
A high frequency ultrasound-coupled fluorescence tomography system, primarily designed for imaging of protoporphyrin IX production in skin tumors in vivo, is demonstrated for the first time. The design couples fiber-based spectral sampling of the protoporphyrin IX fluorescence emission with high frequency ultrasound imaging, allowing thin-layer fluorescence intensities to be quantified. The system measurements are obtained by serial illumination of four linear source locations, with parallel detection at each of five interspersed detection locations, providing 20 overlapping measures of subsurface fluorescence from both superficial and deep locations in the ultrasound field. Tissue layers are defined from the segmented ultrasound images and diffusion theory used to estimate the fluorescence in these layers. The system calibration is presented with simulation and phantom validation of the system in multilayer regions. Pilot in-vivo data are also presented, showing recovery of subcutaneous tumor tissue values of protoporphyrin IX in a subcutaneous U251 tumor, which has less fluorescence than the skin.
一种高频超声耦合荧光层析成像系统，最初设计用于体内皮肤肿瘤中原卟啉 IX 生成的成像，这是首次进行演示。该设计将基于光纤的原卟啉 IX 荧光发射光谱采样与高频超声成像相结合，从而能够定量测量薄层荧光强度。系统测量通过四个线性源位置的连续照明获得，在五个间隔的检测位置中的每一个位置进行平行检测，从而在超声场的浅层和深层位置提供 20 个重叠的次表面荧光测量值。通过分段超声图像定义组织层，并使用扩散理论来估计这些层中的荧光。系统校准通过模拟和多层区域的体模验证进行介绍。还介绍了初步的体内数据，显示了在皮下 U251 肿瘤中恢复的皮下肿瘤组织中原卟啉 IX 的含量，该肿瘤的荧光强度比皮肤低。
