Coulepis A G, Locarnini S A, Ferris A A, Lehmann N I, Gust I D
Intervirology. 1978;10(1):24-31. doi: 10.1159/000148964.
Hepatitis A virus was purified from fecal specimens obtained from 3 patients with naturally acquired hepatitis A, by a process of differential centrifugation, chloroform extraction, column chromatography, and isopycnic ultracentrifugation. Analysis of purified virus by discontinuous SDS-PAGE revealed three major polypeptides with molecular weights of 34,000, 25,500, and 23,000 daltons. These polypeptides appear to be specific for hepatitis A virus and have similar molecular weights to three of the four major polypeptides reported for members of the genus Enterovirus within the family Picornaviridae.
甲型肝炎病毒是从3例自然感染甲型肝炎患者的粪便标本中纯化得到的，纯化过程包括差速离心、氯仿提取、柱色谱和等密度超速离心。通过不连续十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对纯化病毒进行分析，结果显示有三种主要多肽，分子量分别为34,000、25,500和23,000道尔顿。这些多肽似乎是甲型肝炎病毒所特有的，并且分子量与小核糖核酸病毒科肠道病毒属成员所报道的四种主要多肽中的三种相似。
