Coulepis A G, Locarnini S A, Gust I D
J Virol. 1980 Aug;35(2):572-4. doi: 10.1128/JVI.35.2.572-574.1980.
Hepatitis A virus present in the feces of two patients with naturally acquired hepatitis A was purified, radiolabeled with 125I, and analyzed by discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In addition to the three structural polypeptides previously reported, a fourth polypeptide with a molecular weight of 14,000 was detected and shown to be a component of hepatitis A virus by immune precipitation techniques. Intact virions were also shown to sediment at 160S on sucrose gradients. These findings are consistent with hepatitis A virus being an enterovirus within the family Picornaviridae.
从两名自然感染甲型肝炎患者的粪便中提取甲型肝炎病毒，进行纯化，用125I进行放射性标记，然后通过不连续十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分析。除了先前报道的三种结构多肽外，还检测到一种分子量为14,000的第四种多肽，并通过免疫沉淀技术证明它是甲型肝炎病毒的一个组成部分。完整的病毒粒子在蔗糖梯度上也显示在160S处沉降。这些发现与甲型肝炎病毒属于小核糖核酸病毒科肠道病毒的观点一致。
