白花丹和黄茄的体外和体内抗疟活性。
In vitro and in vivo anti-malarial activity of Boerhavia elegans and Solanum surattense.
机构信息
Malaria and Vector Research Group (MVRG), Biotechnology Research Center, Institut Pasteur of Iran, Tehran, Iran.
出版信息
Malar J. 2010 May 12;9:124. doi: 10.1186/1475-2875-9-124.
BACKGROUND
There is an urgent need to identify new anti-malarial drug targets for both prophylaxis and chemotherapy, due to the increasing problem of drug resistance to malaria parasites. In the present study, the aim was to discover novel, effective plant-based extracts for the activity against malaria.
METHODS
Ten plants found in Iran were selected by ethnobotanical survey of medicinal plants. The crude ethanolic extracts were tested for in vitro anti-plasmodial activity against two strains of Plasmodium falciparum: K1 (chloroquine-resistant strain) and CY27 (chloroquine-sensitive strain), using the parasite lactate dehydrogenase (pLDH) assay. The anti-plasmodial activity of the extracts was also assessed in the 4-day suppressive anti-malarial assay in mice inoculated with Plasmodium berghei (ANKA strain). Crude ethanolic extracts showed good anti-plasmodial activity were further fractionated by partitioning in water and dichloromethane.
RESULTS
Of 10 plant species assayed, three species: Boerhavia elegans (Choisy), Solanum surattense (Burm.f.) and Prosopis juliflora (Sw.) showed promising anti-plasmodial activity in vitro (IC50 < or = 50 microg/ml) and in vivo with no toxicity. The dichloromethane fraction of three extracts revealed stronger anti-plasmodial activity than the total extracts.
CONCLUSION
Anti-plasmodial activities of extracts of B. elegans and S. surattense are reported for the first time.
背景
由于疟疾寄生虫对药物的耐药性问题日益严重，迫切需要确定新的抗疟药物靶点，用于预防和化疗。本研究旨在发现针对疟疾具有活性的新型有效植物提取物。
方法
通过对药用植物的民族植物学调查，选择了伊朗的 10 种植物。用寄生虫乳酸脱氢酶（pLDH）测定法，对粗醇提取物进行了体外抗疟原虫活性检测，以评估其对两种疟原虫株（K1[氯喹耐药株]和 CY27[氯喹敏感株]）的活性。粗醇提取物在接种疟原虫伯氏疟原虫（ANKA 株）的小鼠 4 天抑制性抗疟试验中也进行了抗疟活性评估。粗醇提取物显示出良好的抗疟活性，进一步通过在水和二氯甲烷中的分配进行了分离。
结果
在所测定的 10 种植物物种中，有 3 种植物：Boerhavia elegans（Choisy）、Solanum surattense（Burm.f.）和 Prosopis juliflora（Sw.）在体外（IC50 <或= 50 microg/ml）和体内均表现出有希望的抗疟活性，且无毒性。三种提取物的二氯甲烷部分比总提取物具有更强的抗疟活性。
结论
首次报道了 B. elegans 和 S. surattense 提取物的抗疟活性。
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