评价实时聚合酶链反应在人 T 细胞嗜淋巴细胞病毒 1 和 2 作为献血者筛查的确认试验中的应用。
Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors.
机构信息
Minas Gerais State Blood Center, Belo Horizonte, MG, Brazil.
出版信息
Rev Soc Bras Med Trop. 2010 Mar-Apr;43(2):111-5. doi: 10.1590/s0037-86822010000200001.
INTRODUCTION
HTLV-1/2 screening among blood donors commonly utilizes an enzyme-linked immunosorbent assay (EIA), followed by a confirmatory method such as Western blot (WB) if the EIA is positive. However, this algorithm yields a high rate of inconclusive results, and is expensive.
METHODS
Two qualitative real-time PCR assays were developed to detect HTLV-1 and 2, and a total of 318 samples were tested (152 blood donors, 108 asymptomatic carriers, 26 HAM/TSP patients and 30 seronegative individuals).
RESULTS
The sensitivity and specificity of PCR in comparison with WB results were 99.4% and 98.5%, respectively. PCR tests were more efficient for identifying the virus type, detecting HTLV-2 infection and defining inconclusive cases.
CONCLUSIONS
Because real-time PCR is sensitive and practical and costs much less than WB, this technique can be used as a confirmatory test for HTLV in blood banks, as a replacement for WB.
简介
在血液筛查中，通常使用酶联免疫吸附试验（EIA）检测 HTLV-1/2，若 EIA 阳性，则使用免疫印迹法（WB）进行确认。然而，这种算法会导致大量不确定结果，且费用昂贵。
方法
我们开发了两种用于检测 HTLV-1 和 2 的实时定量 PCR 检测方法，并对 318 份样本（152 份献血者、108 名无症状携带者、26 名 HAM/TSP 患者和 30 名血清阴性个体）进行了检测。
结果
与 WB 结果相比，PCR 的灵敏度和特异性分别为 99.4%和 98.5%。PCR 检测在确定病毒类型、检测 HTLV-2 感染和定义不确定结果方面更有效。
结论
由于实时 PCR 灵敏且实用，成本远低于 WB，因此，该技术可用于血库中 HTLV 的确认检测，替代 WB。
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