单细胞分辨率下复杂组织的荧光/发光昼夜节律成像。
Fluorescence/luminescence circadian imaging of complex tissues at single-cell resolution.
作者信息
Sellix Michael T, Currie Jake, Menaker Michael, Wijnen Herman
出版信息
J Biol Rhythms. 2010 Jun;25(3):228-32. doi: 10.1177/0748730410368016.
Abstract
The use of luciferase reporter genes together with luminescence detection has enabled high frequency monitoring of molecular circadian clock function in living tissues. With the help of an intensified CCD camera combined with an inverted epifluorescence microscope, the authors have established a new imaging strategy that makes use of transgenic cell type-specific expression of fluorescent proteins to identify cells of interest for subsequent circadian luminescence recording at single-cell resolution.
摘要
荧光素酶报告基因与发光检测的结合使用，使得对活体组织中分子生物钟功能进行高频监测成为可能。借助与倒置落射荧光显微镜相结合的增强型电荷耦合器件相机，作者建立了一种新的成像策略，该策略利用荧光蛋白的转基因细胞类型特异性表达来识别感兴趣的细胞，以便随后以单细胞分辨率进行昼夜发光记录。
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本文引用的文献
1
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Neuron. 2009 Oct 29;64(2):251-66. doi: 10.1016/j.neuron.2009.08.026.
2
The neuropeptide pigment-dispersing factor adjusts period and phase of Drosophila's clock.神经肽色素分散因子可调节果蝇生物钟的周期和相位。
J Neurosci. 2009 Feb 25;29(8):2597-610. doi: 10.1523/JNEUROSCI.5439-08.2009.
3
The lateral and dorsal neurons of Drosophila melanogaster: new insights about their morphology and function.黑腹果蝇的外侧神经元和背侧神经元：关于其形态与功能的新见解
Cold Spring Harb Symp Quant Biol. 2007;72:517-25. doi: 10.1101/sqb.2007.72.063.
4
Use of firefly luciferase activity assays to monitor circadian molecular rhythms in vivo and in vitro.使用萤火虫荧光素酶活性测定法在体内和体外监测昼夜节律分子节律。
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5
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6
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7
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