苹果皮多酚抗幽门螺杆菌的体内外作用。

In vitro and in vivo effects of apple peel polyphenols against Helicobacter pylori.

机构信息

Laboratory of Pharmacognosy, Department of Pharmacy, Faculty of Pharmacy, Universidad de Concepción, Concepción, Chile.

出版信息

J Agric Food Chem. 2010 Jun 23;58(12):7172-9. doi: 10.1021/jf100274g.

PMID:20486708

Abstract

The inhibitory effects of a standarized apple peel polyphenol-rich extract (APPE) against Helicobacter pylori infection and vacuolating bacterial toxin (VacA) induced vacuolation were investigated. Apple peel polyphenols significantly prevented vacuolation in HeLa cells with an IC(50) value of 390 microg of gallic acid equivalents (GAE)/mL. APPE also displayed an in vitro antiadhesive effect against H. pylori. A significant inhibition was observed with a 20-60% reduction of H. pylori attachment at concentrations between 0.250 and 5 mg of GAE/mL. In a short-term infection model (C57BL6/J mice), two levels of APPE doses (150 and 300 mg/kg/day) showed an inhibitory effect on H. pylori attachment. Orally administered apple peel polyphenols also showed an anti-inflammatory effect on H. pylori-associated gastritis, lowering malondialdehyde levels and gastritis scores.

摘要

本研究旨在探讨标准化苹果皮多酚提取物（APPE）对幽门螺杆菌感染和空泡毒素（VacA）诱导空泡形成的抑制作用。结果表明，苹果皮多酚对 HeLa 细胞空泡化具有显著的抑制作用，其半数抑制浓度（IC50）值为 390μg 没食子酸当量（GAE）/mL。此外，APPE 还表现出体外抗幽门螺杆菌黏附的作用，在 0.250-5mg GAE/mL 浓度范围内，对幽门螺杆菌的黏附抑制率可达 20%-60%。在短期感染模型（C57BL6/J 小鼠）中，两个剂量水平的 APP（150 和 300mg/kg/d）对幽门螺杆菌的黏附具有抑制作用。口服给予苹果皮多酚也对幽门螺杆菌相关性胃炎表现出抗炎作用，降低丙二醛水平和胃炎评分。