精子相关抗原 8（SPAG8），在精子发生过程中睾丸中 CREM 激活物的新型调节因子。
Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.
机构信息
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, China.
出版信息
FEBS Lett. 2010 Jul 2;584(13):2807-15. doi: 10.1016/j.febslet.2010.05.016. Epub 2010 May 17.
cAMP response element modulator (CREM)-mediated gene expression is an essential regulatory mechanism for germ cell differentiation. CREM and its coactivator in testis, ACT, activate the transcription of many essential genes for spermatogenesis. Sperm associated antigen 8 (SPAG8) is a testis-specific component that is expressed during germ cell differentiation. In this study, we found the pattern of SPAG8 expression largely overlapped with that of ACT during spermatogenesis and verified the association of SPAG8 with ACT. Furthermore, we showed that SPAG8 enhanced the transcriptional activation of ACT-mediated CREMtau by strengthening the binding of ACT to CREMtau. These results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis.
环磷酸腺苷反应元件结合蛋白介导体（CREM）介导的基因表达是生殖细胞分化的重要调节机制。CREM 及其在睾丸中的共激活因子 ACT 激活许多精子发生所必需基因的转录。精子相关抗原 8（SPAG8）是一种睾丸特异性成分，在生殖细胞分化过程中表达。在本研究中，我们发现 SPAG8 的表达模式在很大程度上与精子发生过程中的 ACT 表达模式重叠，并验证了 SPAG8 与 ACT 的关联。此外，我们表明 SPAG8 通过增强 ACT 与 CREMtau 的结合来增强 ACT 介导的 CREMtau 的转录激活。这些结果表明 SPAG8 作为 ACT 的调节剂，在精子发生过程中 CREM-ACT 介导的基因转录中发挥重要作用。
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