Microbiology Service, Hospital Donostia, Paseo Dr Beguiristain s/n, San Sebastián, Spain.
Eur J Clin Microbiol Infect Dis. 2010 Aug;29(8):955-60. doi: 10.1007/s10096-010-0951-x. Epub 2010 May 21.
To describe the circulation dynamics of human rotavirus genotypes in a region of southern Europe over a 13-year period. The G- and P-types of rotavirus isolates of patients aged less than 5 years were analyzed using multiplex, reverse transcription polymerase-chain reaction. Of 1,538 isolates investigated, a combination of individual G- and P-types was obtained in 1,368. The most prevalent combination was G1[P8] (57.5% of the genotyped strains), which circulated in all seasons and predominated in nine out of 13 seasons. The strains G2[P4] (14.4%), G3[P8] (8.3%), G4[P8] (5.5%) and G9[P8] (13.4%) circulated intermittently. G4[P8] strains were frequently detected in the 1990s but only sporadically after 2000. G9[P8] strains emerged from 1997-1998 and became dominant in the winters of 2005-2007. G2[P4] strains were predominant in 2003-2004, before the rotavirus vaccines were commercialized. Unusual combinations of common G- and P-types and the presence of unusual G- and/or P-types (G6[P14], G8[P8], G8[P14] and G12[P8]) were rarely observed (<1%). We found no differences in hospitalization due to distinct genotypes. G-types G1-G4 and G9 represented >99% of circulating rotaviruses over a 13-year period. Therefore, vaccine efficacy in this region can be expected to be high.
描述 13 年来欧洲南部地区人类轮状病毒基因型的循环动态。使用多重反转录聚合酶链反应分析了年龄小于 5 岁的患者的轮状病毒分离株的 G 型和 P 型。在调查的 1538 个分离株中，有 1368 个分离株获得了个体 G 型和 P 型的组合。最常见的组合是 G1[P8]（57.5%的基因分型株），它在所有季节传播，并在 13 个季节中的 9 个季节中占优势。G2[P4]（14.4%）、G3[P8]（8.3%）、G4[P8]（5.5%）和 G9[P8]（13.4%）的菌株间歇性传播。G4[P8]株在 20 世纪 90 年代经常检测到，但 2000 年后很少发现。G9[P8]株于 1997-1998 年出现，并在 2005-2007 年冬季成为优势株。G2[P4]株在轮状病毒疫苗商业化之前的 2003-2004 年占优势。常见 G 型和 P 型的不常见组合以及不常见 G 型和/或 P 型（G6[P14]、G8[P8]、G8[P14]和 G12[P8]）的存在很少观察到（<1%）。我们没有发现不同基因型导致住院的差异。G 型 G1-G4 和 G9 在 13 年内代表了循环轮状病毒的>99%。因此，可以预期该地区的疫苗有效性会很高。
