Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
J Zhejiang Univ Sci B. 2010 Jun;11(6):437-42. doi: 10.1631/jzus.B0900402.
Ultrafiltration and a series of chromatographic steps were used to isolate and purify polysaccharides from Tremella aurantialba fruit bodies. Three crude fractions (TAP50w, TAP10-50w, and TAP1-10w), five semi-purified fractions (TAPA-TAPE), and one purified fraction (TAPA1) were obtained. A sulfated derivative of TAPA1 (TAPA1-s) was prepared by chemical modification. The immunostimulating activity of the polysaccharide fractions in vitro was determined using the mouse spleen lymphocyte proliferation assay. Of the three crude fractions tested, cell proliferation rates were increased most by TAP50w. Furthermore, TAPA1-s was markedly more stimulatory than TAPA1, indicating that sulfonation was an effective way to enhance the immunostimulating activity of polysaccharide.
采用超滤和一系列层析步骤从银耳子实体中分离和纯化多糖。得到了三个粗提物(TAP50w、TAP10-50w 和 TAP1-10w)、五个半纯化物(TAPA-TAPE)和一个纯化物(TAPA1)。通过化学修饰制备了 TAPA1 的硫酸化衍生物(TAPA1-s)。通过小鼠脾淋巴细胞增殖试验测定了多糖级分的体外免疫刺激活性。在所测试的三个粗提物中,TAP50w 对细胞增殖率的促进作用最大。此外,TAPA1-s 比 TAPA1 更具刺激性,表明硫酸化是增强多糖免疫刺激活性的有效方法。