Division of Cardiovascular Medicine, Department of Medicine, Gerald McGinnis Cardiovascular Institute, Allegheny-Singer Research Institute, West-Penn Allegheny Health System, Pittsburgh, PA 15212-4772, USA.
Gene Ther. 2010 Nov;17(11):1318-24. doi: 10.1038/gt.2010.86. Epub 2010 May 27.
We report a non-viral gene transfer method using ultrasound induced microbubble destruction to allow the uptake of plasmid gene transfer vectors to the cells of the mouse salivary gland. The Luciferase (Luc) reporter gene, driven by a cytomegalovirus (CMV) promoter, was delivered unilaterally to the submandibular salivary gland via retroductal cannulation and Luc expression was monitored with in vivo imaging. The CMV-Luc plasmid was delivered to the salivary gland in a carrier solution containing microbubbles composed of lipid-encased perfluoropropane gas, with two different concentrations of microbubbles used (100 and 15% volume/volume). An Adenoviral (Ad) vector using an identical CMV-Luc expression cassette was used as a positive control at two different dosages. Whereas ultrasound-assisted gene transfer (UAGT) with 100% microbubbles was weak and rapidly extinguished, UAGT with the 15% microbubble solution was robust and stable for 28 days. UAGT seems to be a practicable and promising method for non-viral gene delivery to the salivary glands.
我们报告了一种使用超声诱导微泡破坏的非病毒基因转移方法，使质粒基因转移载体能够被摄取到小鼠唾液腺细胞中。荧光素酶（Luc）报告基因由巨细胞病毒（CMV）启动子驱动，通过逆行导管插入单侧下颌唾液腺，并通过体内成像监测 Luc 表达。CMV-Luc 质粒在含有由脂质包裹的全氟丙烷气体组成的微泡的载体溶液中递送至唾液腺，使用了两种不同浓度的微泡（100%和 15%体积/体积）。使用相同的 CMV-Luc 表达盒的腺病毒（Ad）载体作为阳性对照，使用了两种不同的剂量。虽然 100%微泡的超声辅助基因转移（UAGT）较弱且迅速消失，但 15%微泡溶液的 UAGT 强大且稳定 28 天。UAGT 似乎是一种可行且有前途的非病毒基因传递方法，适用于唾液腺。
