Department of Military and Emergency Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Chem Biol Interact. 2010 Aug 5;186(3):275-9. doi: 10.1016/j.cbi.2010.05.010. Epub 2010 May 27.
We previously reported that lipid-soluble quercetin, not water-soluble dihydroquercetin, protects human red blood cells against oxidative damage. The objectives of this study were to determine if an antihemolytic effect could be produced by other lipid-soluble antioxidants and if anti-inflammatory activity played a role in antihemolysis by quercetin. This study compared three lipid-soluble polyphenols, muscadine, curcumin and quercetin, and three lipid- (alpha-tocopherol and alpha-tocotrienol) or water-soluble (ascorbic acid) vitamins. Among the tested polyphenols, muscadine was the most potent in inhibiting superoxide and 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH)-generated peroxyl radicals, whereas ascorbic acid was the most potent inhibitor of hydrogen peroxide. Activities of the polyphenols after lipid extractions showed that curcumin inhibited superoxide production to a greater extent than quercetin and muscadine. All blood cells were tested 20 min after incubation with the selected compounds. All the polyphenols caused inhibition of N-formyl-l-methionyl-l-leucyl-l-phenylalanine-induced neutrophil oxidative bursts. Quercetin, but not other polyphenols, significantly reduced AAPH-induced oxidative hemolysis. No significant effect on neutrophil oxidative burst or oxidative hemolysis was found with any of the tested vitamins. These results suggest that quercetin enhances the resistance of membrane to destruction by free radicals. This effect of quercetin is not directly mediated through antioxidative or anti-inflammatory actions. Antioxidant or anti-inflammatory potency may not be used as a simple criterion to select polyphenols for cell protection benefits.
我们之前曾报道脂溶性槲皮素而非水溶性二氢槲皮素可保护人红细胞免受氧化损伤。本研究的目的是确定其他脂溶性抗氧化剂是否具有抗溶血作用，以及槲皮素的抗溶血作用是否与抗炎活性有关。本研究比较了三种脂溶性多酚（麝香葡萄、姜黄素和槲皮素）以及三种脂溶性维生素（α-生育酚和α-生育三烯酚）和三种水溶性维生素（抗坏血酸）。在所测试的多酚中，麝香葡萄对超氧阴离子和 2,2-偶氮双（2-脒基丙烷）二盐酸盐（AAPH）生成的过氧自由基的抑制作用最强，而抗坏血酸对过氧化氢的抑制作用最强。脂质提取后多酚的活性表明，姜黄素对超氧阴离子的抑制作用强于槲皮素和麝香葡萄。在与选定化合物孵育 20 分钟后，测试所有血细胞。所有多酚均能抑制 N-甲酰基-L-甲硫氨酸-L-亮氨酸-L-苯丙氨酸诱导的中性粒细胞氧化爆发。槲皮素，但不是其他多酚，可显著降低 AAPH 诱导的氧化溶血。测试的维生素对中性粒细胞氧化爆发或氧化溶血均无明显影响。这些结果表明，槲皮素增强了膜对自由基破坏的抵抗力。槲皮素的这种作用不是通过抗氧化或抗炎作用直接介导的。抗氧化或抗炎效力不能简单地用作选择用于细胞保护益处的多酚的标准。
